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  1. Induction and detection of immunoglobulin class switching During the immune response, B cells stimulated by different antigens can switch to express different heavy chain constant region genes and change the type of im
  2. B Cell activation B cells are activated and proliferate upon activation by specific antigens, anti-Ig antibodies, and/or mitogens.
  3. B Identification of functional subpopulations of cells Peripheral mature B cells can be divided into several subpopulations. Traditionally, B cells have been divided into two subpopulations, CD5+ B1 cells and CD5 B2
  4. Experimental effects of anticancer drugs on ⅡDNA topoisomerase The effect of anticancer drugs on DNA topoisomerase activity assay was performed using agarose gel electrophoresis to determine the effect of anticancer drugs o
  5. Isolation and purification of IgG IgG isolation and purification experiments can be used to: prepare enzyme labeled antibodies or fluorescent antibodies.
  6. Preparation of EBV-transformed B cells Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
  7. Detection of NK/LAK cell killing activity Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
  8. In vivo induction and detection of antibodies Detection of Antibody Production in Vivo by ELISA by J.E. Colligan et al, Translated by Xuitao Cao et al. This experiment is from the "Compendium of Immunology
  9. ELISA for detection of antibodies This method detects IgG, I g M and IgA in serum or cell culture supernatants (Fig. 8. 1 0 . 1 ). The color of the liquid in the assay wells is directly proporti
  10. B Detection of cell-secreted antibodies The following is an example of mitogen-induced antibody production by B cells using American continental protein. This method has been adapted for use with a va
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