Macrophage activation goes through two phases: a sensitization phase and an activation phase. While most protocols for macrophage activation use a one-step appr
To provide a phenotypic assay for human monocytes/macrophages.
Author: J.E. Colligan et al, Translator: Xuitao Cao et al. This experiment is from the "Comprehen
This unit describes the use of 4-color fluorescence to analyze the levels of IFN-7, IL-4 and IL-2 expressed in CD4+ T cells.
(can be used to calculate T h l and
Flow cytometry is used to analyze membrane surface antigens and intracellular antigens expressed by cells, and is capable of specifically distinguishing differe
Lipid rafts are important in immunology because T-cell receptors and several other important T-cell signaling proteins are present in lipid rafts or are recruit
Antibodies against phosphopeptides recognize proteins that are only in the phosphorylated state. The antibody does not cross-react with unphosphorylated protein
This module introduces the mouse T lymphocyte proliferation assay.
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from the "Comp
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