Protocols

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  1. Selection and preparation of antigen-presenting cells Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
  2. Activation of mouse macrophages Macrophage activation goes through two phases: a sensitization phase and an activation phase. While most protocols for macrophage activation use a one-step appr
  3. Phenotyping of Human Monocytes/Macrophages To provide a phenotypic assay for human monocytes/macrophages. Author: J.E. Colligan et al, Translator: Xuitao Cao et al. This experiment is from the "Comprehen
  4. Detection of intracellular factors by flow cytometry This unit describes the use of 4-color fluorescence to analyze the levels of IFN-7, IL-4 and IL-2 expressed in CD4+ T cells. (can be used to calculate T h l and
  5. Immunofluorescence and cell separation Flow cytometry is used to analyze membrane surface antigens and intracellular antigens expressed by cells, and is capable of specifically distinguishing differe
  6. Separation and application of lipid rafts Lipid rafts are important in immunology because T-cell receptors and several other important T-cell signaling proteins are present in lipid rafts or are recruit
  7. Preparation of antibodies recognizing specific tyrosine phosphorylated polypeptides Antibodies against phosphopeptides recognize proteins that are only in the phosphorylated state. The antibody does not cross-react with unphosphorylated protein
  8. Detection of apoptosis and other forms of cell death Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
  9. T lymphocyte proliferation assay This module introduces the mouse T lymphocyte proliferation assay. Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from the "Comp
  10. B Early events in lymphocyte activation Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
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