Protocols

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  1. Detection of human lymphocytes in whole blood by flow cytometry This method analyzes whole blood cells compared to analyzing Ficoll-isolated single nucleated cells, thus taking less time, requiring a smaller sample size, and
  2. Isolation of B-cell subpopulations by immunomagnetic bead assay Anti-CD 19 labeled B cells, which have relatively low signal intensity, show mitogenic and antibody secretory responses similar to those obtained by negative sc
  3. Presentation of exogenous antigens to T cells For granular antigens or liposome-encapsulated antigens, macrophages were applied as antigen-presenting cells. Author: J.E. Colligan et al, Translator: Xuitao C
  4. Isolation and expansion of human NK T cells This protocol allows the identification and quantitative analysis of trace amounts of N K T cells. Effective measures should be taken to block non-specific stai
  5. Detection of Fcγ Receptor-Mediated Adhesion and Phagocytosis Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
  6. Detection of anti-tumor activity of macrophages Mouse macrophage cell lines with tumor cell killing activity, such as A N A -1 and G G 2E E . Mouse primary macrophages can be freshly isolated. Author: J.E. Co
  7. Detection of phagocytosis and killing function of macrophages An assay for macrophage phagocytosis and killing function. Author: J.E. Colligan et al, Translator: Xuitao Cao et al. This experiment is from "Compendium Immuno
  8. Detection of Nitric Oxide in Macrophages This protocol reflects the level of nitric oxide secretion by detecting nitrite in the culture supernatants of activated macrophages (walled or non-walled) Auth
  9. Phenotypic assay of mouse macrophages Due to the lack of characteristic surface markers of macrophages or macrophages at different stages of differentiation, macrophages are currently identified bas
  10. Isolation of mouse macrophages Introduction of a method for the isolation of mouse macrophages by J.E. Colligan et al, translated by Xuitao Cao et al. This experiment is from the "Compendium
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