The cells used in the assay are mouse L 929 fibroblasts, which have a high sensitivity to interferon and V S V . The cells should be derived from laboratories t
Enzyme-linked immunospot test (ELISPOT) is based on the principle that cells that secrete certain protein molecules (e.g. cytokines) are able to secrete a relat
This protocol is for the detection of cytokine production by activated antigen-specific T cells in vitro. If other stimulants or stimulated cells are used in vi
Newly synthesized IL-18 is a precursor molecule to the signal peptide, which is enzymatically cleaved in order to activate it. In this ELISA method, in order to
The ELISA method can be used to detect human IL-15 in culture supernatants and serum samples. This method can also be used for the detection of monkey IL-15, bu
ELISA is highly specific for human or mouse IL-13 and does not recognize other cytokines in the sample. Author: J.E. Colligan et al, Translator: Xuitao Cao et a
This is a method for the specific detection of IL-12 heterodimers. Since IL-12 p4 0 monomer cannot be detected, this method is able to detect IL-12 p7 5 heterod
This protocol uses an antibody specific for mouse IL- 1 0 in the E LISA method to detect mouse IL- 1 0 , and does not detect human or viral IL-10. IL-10 from ot
A method is described for the detection and quantification of one of the cytokines in the presence of the other using neutralizing antibodies to murine IL-2 and
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