dITP (100mM)

Item Number

D745313

• Synonyms: [hydroxy-[hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(6-oxo-3H-purin-9-yl)oxolan-2-yl]methoxy]phosphoryl]oxy-phosphoryl]oxyphosphonicaci | 2'-Deoxyinosinetriphosphate | 2'-DEOXYINOSINE-5'-TRIPHOSPHATE | 2'-Deoxyinosine-5'-triphosphoricacid | 2'-Deoxyinosine5'-triph
• Specifications & Purity: sterile, BioReagent, Suitable for molecular biology, PCR Reagent, DNase, RNase free, for DNA and RNA applications, ≥99%, 100mM
• Stability And Storage: Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
• Storage Temp: Store at -20°C,Avoid repeated freezing and thawing
• Shipped In: Ice chest + Ice pads
• Grade: BioReagent, DNase, RNase free, for DNA and RNA applications, PCR Reagent, sterile, 适用于分子生物学

Product Description

dITP, 2'-deoxy-inosine-5'-triphosphate, can be used in PCR, real-time PCR, RT-PCR, cDNA synthesis, as well as primer extension and SNP genotyping with Taq DNA polymerase. It is generally not used in PCR with proofreading DNA polymerase.dITP cannot replace dGTP completely in PCR mix. Otherwise, DNA amplification will be inhibited significantly. It is recommended to replace10% dGTP by dITP in PCR mix. For example, dNTP/dITP Mixture (2.5mM each + 0.25mM dITP) can be used in PCR with high fidelity DNA polymerase .The molecular formula of dITP is C10H12N4O13P3Na3 with a molecular weight of 558.11 (492.1 in acid form).This dITP solution is prepared with ultrapure water to a concentration of 100mM. The pH is titrated to 7.0-7.5 with high-purity NaOH solution.This product is free of DNase, RNase, phosphatase and protease, and can be directly used for different molecular biology applications.

Precautions：

This product can be thawed at room temperature. Keep it on ice for use and store at -20℃ immediately after use.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

None