dNTP/dUTP Mixture (2.5mM each/5mM)

Item Number

D745378

• Synonyms: dNTP/dUTP Mixture | dNTP/dUTP Mix
• Specifications & Purity: BioReagent, Suitable for molecular biology, PCR Reagent, DNase, RNase free, sterile, for DNA and RNA applications, ≥99%, dATP、dCTP、dGTP、dTTP: 2.5mM dUTP: 5mM
• Stability And Storage: Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
• Storage Temp: Store at -20°C,Avoid repeated freezing and thawing
• Shipped In: Ice chest + Ice pads
• Grade: BioReagent, DNase, RNase free, for DNA and RNA applications, PCR Reagent, sterile, 适用于分子生物学

Product Description

This dNTP/dUTP Mixture (2.5mM each/5mM) is a mixed aqueous solution of dATP, dCTP, dGTP, dTTP and dUTP, where the concentration of dATP, dCTP and dGTP is 2.5mM and the concentration of dUTP is 5mM. This product is commonly used in PCR with Taq DNA polymerase, primer extension and cDNA synthesis. It is more commonly used in conjunction with the UDG enzyme that can recognize and excise deoxyuracil bases to prevent carryover contamination by PCR products from surroundings. This product can be used in PCR with polymerase family A members such as Taq, and also for isothermal amplification with Bst DNA Polymerase. Some enzymes that can use dATP, dTTP, dGTP, dCTP, may not be able to use dUTP. Please refer to manufacture’s instructions of a particular enzyme or make a test.Usually, this product and uracil DNA glycosylase (UDG enzyme) are used in conjunction to establish the dUTP/UDG anti-pollution system in which the UDG enzyme can enzymatically remove possible contamination from previous PCR products containing deoxyuracil (dU), thus effectively minimize cross-over contamination.This dNTP/dUTP Mixture is prepared with ultrapure water and the pH is titrated to 7.0-7.5 with high-purity NaOH solution.This product is free of DNase and RNase, can be directly used for different molecular biology applications.
Precautions：
The UDG enzyme does not work for cases that have been contaminated by dU-free PCR products. In this case, the primers can be redesigned to amplify other regions of target DNA. The dUTP/UDG anti-pollution system should be established to minimize cross-over contamination.This product can be thawed at room temperature. Keep it on ice for use and store at -20℃ immediately after use.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.
Instructions for Use：
None