Zwitterionic, both positively and negatively charged. Utilized for applications ranging from solubilizing membrane proteins in non-denaturing conditions to creating denaturing conditions during gel electrophoresis.
Application：
CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry.
Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.
1.Li Guanglei, Chen Yao, Liu Fei, Bi Wenhua, Wang Chenxin, Lu Danfeng, Wen Dan. (2023) Portable visual and electrochemical detection of hydrogen peroxide release from living cells based on dual-functional Pt-Ni hydrogels. Microsystems & Nanoengineering, 9 (1):(1-10). [PMID:38033990][10.1038/s41378-023-00623-y]
2.Xu Daokun, Huang Haolun, Liu Zhen, Wang Yumei, Liu Qinan, Jiang Xing, Yang Jun, Ling Rui. (2023) Comprehensive characterization and detection of nut allergens in bakery foods using Q–TOF mass spectrometry and bioinformatics. Food Quality and Safety, (1):[PMID:11741838][10.1093/fqsafe/fyad061]
3.Jie Zhao, Shuaiqian Wang, Diandian Jiang, Yan Lu, Yu Chen, Yong Tang, Jie Tang, Zhenju Jiang, Hongbin Lin, Wei Dong. (2023) Unravelling the interaction between α-SOH and myofibrillar protein based on spectroscopy and molecular dynamics simulation. Food Chemistry-X, 20 (100986). [PMID:38144868][10.1016/j.fochx.2023.100986]
4.Zhe Chen, Yunxia Wang. (2023) A label- and enzyme-free fluorescence assay based on thioflavin T–induced G-quadruplexes for the detection of telomerase activity. JOURNAL OF CHEMICAL RESEARCH, [PMID:][10.1177/17475198221139085]
5.Pengfei Dong, Longyi Zhu, Jing Huang, Jujie Ren, Jianping Lei. (2019) Electrocatalysis of cerium metal-organic frameworks for ratiometric electrochemical detection of telomerase activity. BIOSENSORS & BIOELECTRONICS, 138 (111313). [PMID:31108380][10.1016/j.bios.2019.05.018]
6.Min Zhang, Chong-sheng Peng, Xiao-bo Li. (2017) Human intestine and liver microsomal metabolic differences between C19-diester and monoester diterpenoid alkaloids from the roots of Aconitum carmichaelii Debx.. TOXICOLOGY IN VITRO, 45 (318). [PMID:28919359][10.1016/j.tiv.2017.09.011]
7.Feifei Gong, Huaxin Yang, Wen Sun, Junzi Cao, Wanhui Liu. (2016) Development and validation of a micellar electrokinetic capillary chromatography method for the determination of goserelin and related substances. ELECTROPHORESIS, 37 (4):(623-629). [PMID:26635325][10.1002/elps.201500328]
8.Yang Li,Ying Peng,Mengyue Wang,Pengfei Tu,Xiaobo Li. (2017-08-05) Human Gastrointestinal Metabolism of the Cistanches Herba Water Extract in Vitro: Elucidation of the Metabolic Profile Based on Comprehensive Metabolite Identification in Gastric Juice, Intestinal Juice, Human Intestinal Bacteria, and Intestinal Microsomes.. Journal of agricultural and food chemistry, 65 ((34)):(7447-7456). [PMID:28771352]
References
1.Li Guanglei, Chen Yao, Liu Fei, Bi Wenhua, Wang Chenxin, Lu Danfeng, Wen Dan. (2023) Portable visual and electrochemical detection of hydrogen peroxide release from living cells based on dual-functional Pt-Ni hydrogels. Microsystems & Nanoengineering, 9 (1):(1-10). [PMID:38033990][10.1038/s41378-023-00623-y]
2.Xu Daokun, Huang Haolun, Liu Zhen, Wang Yumei, Liu Qinan, Jiang Xing, Yang Jun, Ling Rui. (2023) Comprehensive characterization and detection of nut allergens in bakery foods using Q–TOF mass spectrometry and bioinformatics. Food Quality and Safety, (1):[PMID:11741838][10.1093/fqsafe/fyad061]
3.Jie Zhao, Shuaiqian Wang, Diandian Jiang, Yan Lu, Yu Chen, Yong Tang, Jie Tang, Zhenju Jiang, Hongbin Lin, Wei Dong. (2023) Unravelling the interaction between α-SOH and myofibrillar protein based on spectroscopy and molecular dynamics simulation. Food Chemistry-X, 20 (100986). [PMID:38144868][10.1016/j.fochx.2023.100986]
4.Zhe Chen, Yunxia Wang. (2023) A label- and enzyme-free fluorescence assay based on thioflavin T–induced G-quadruplexes for the detection of telomerase activity. JOURNAL OF CHEMICAL RESEARCH, [PMID:][10.1177/17475198221139085]
5.Pengfei Dong, Longyi Zhu, Jing Huang, Jujie Ren, Jianping Lei. (2019) Electrocatalysis of cerium metal-organic frameworks for ratiometric electrochemical detection of telomerase activity. BIOSENSORS & BIOELECTRONICS, 138 (111313). [PMID:31108380][10.1016/j.bios.2019.05.018]
6.Min Zhang, Chong-sheng Peng, Xiao-bo Li. (2017) Human intestine and liver microsomal metabolic differences between C19-diester and monoester diterpenoid alkaloids from the roots of Aconitum carmichaelii Debx.. TOXICOLOGY IN VITRO, 45 (318). [PMID:28919359][10.1016/j.tiv.2017.09.011]
7.Feifei Gong, Huaxin Yang, Wen Sun, Junzi Cao, Wanhui Liu. (2016) Development and validation of a micellar electrokinetic capillary chromatography method for the determination of goserelin and related substances. ELECTROPHORESIS, 37 (4):(623-629). [PMID:26635325][10.1002/elps.201500328]
8.Yang Li,Ying Peng,Mengyue Wang,Pengfei Tu,Xiaobo Li. (2017-08-05) Human Gastrointestinal Metabolism of the Cistanches Herba Water Extract in Vitro: Elucidation of the Metabolic Profile Based on Comprehensive Metabolite Identification in Gastric Juice, Intestinal Juice, Human Intestinal Bacteria, and Intestinal Microsomes.. Journal of agricultural and food chemistry, 65 ((34)):(7447-7456). [PMID:28771352]
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E2417142
![3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfate (CHAPS) - 10mM in DMSO, used for , CAS No. 75621-03-3, by Aladdin Scientific](https://staging.aladdinsci.com/media/catalog/product/cache/7c7539e41aff5deaa39728f000f29ebe/C/4/C425843.png)
