CHAPS is a zwitterionic nondenaturing detergent for solubilizing membrane proteins.
Storage Temp
Store at 2-8°C,Argon charged
Shipped In
Wet ice
Product Description
CHAPS is a non-denaturing, zwitterionic detergent derived from cholic acid. CHAPS is often used as a detergent in the solubilization and purification of membrane proteins for several advantageous reasons. CHAPS detergent is non-denaturing to membrane proteins, can solubilize proteins, disaggregate protein-protein interactions and is electrically neutral. CHAPS is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH 2 to 12. The critical micelle concentration of CHAPS is 6-10mM. A non-denaturing, zwitterionic detergent for membrane proteins
Application：
Buffer; zwitterionic nondenaturing detergent for solubilizing membrane proteins CHAPS is used in the laboratory to dissolve biological macromolecules such as proteins. It acts as a non-denaturing solvent used in purification of membrane proteins. It is also useful in conjunction with nonionic detergents like Triton X-100. Further, it is used as a non-denaturing zwitterionic detergent for membrane biochemistry.
1.Li Guanglei, Chen Yao, Liu Fei, Bi Wenhua, Wang Chenxin, Lu Danfeng, Wen Dan. (2023) Portable visual and electrochemical detection of hydrogen peroxide release from living cells based on dual-functional Pt-Ni hydrogels. Microsystems & Nanoengineering, 9 (1):(1-10). [PMID:38033990][10.1038/s41378-023-00623-y]
2.Xu Daokun, Huang Haolun, Liu Zhen, Wang Yumei, Liu Qinan, Jiang Xing, Yang Jun, Ling Rui. (2023) Comprehensive characterization and detection of nut allergens in bakery foods using Q–TOF mass spectrometry and bioinformatics. Food Quality and Safety, (1):[PMID:11741838][10.1093/fqsafe/fyad061]
3.Jie Zhao, Shuaiqian Wang, Diandian Jiang, Yan Lu, Yu Chen, Yong Tang, Jie Tang, Zhenju Jiang, Hongbin Lin, Wei Dong. (2023) Unravelling the interaction between α-SOH and myofibrillar protein based on spectroscopy and molecular dynamics simulation. Food Chemistry-X, 20 (100986). [PMID:38144868][10.1016/j.fochx.2023.100986]
4.Zhe Chen, Yunxia Wang. (2023) A label- and enzyme-free fluorescence assay based on thioflavin T–induced G-quadruplexes for the detection of telomerase activity. JOURNAL OF CHEMICAL RESEARCH, [PMID:][10.1177/17475198221139085]
5.Pengfei Dong, Longyi Zhu, Jing Huang, Jujie Ren, Jianping Lei. (2019) Electrocatalysis of cerium metal-organic frameworks for ratiometric electrochemical detection of telomerase activity. BIOSENSORS & BIOELECTRONICS, 138 (111313). [PMID:31108380][10.1016/j.bios.2019.05.018]
6.Min Zhang, Chong-sheng Peng, Xiao-bo Li. (2017) Human intestine and liver microsomal metabolic differences between C19-diester and monoester diterpenoid alkaloids from the roots of Aconitum carmichaelii Debx.. TOXICOLOGY IN VITRO, 45 (318). [PMID:28919359][10.1016/j.tiv.2017.09.011]
7.Feifei Gong, Huaxin Yang, Wen Sun, Junzi Cao, Wanhui Liu. (2016) Development and validation of a micellar electrokinetic capillary chromatography method for the determination of goserelin and related substances. ELECTROPHORESIS, 37 (4):(623-629). [PMID:26635325][10.1002/elps.201500328]
8.Yang Li,Ying Peng,Mengyue Wang,Pengfei Tu,Xiaobo Li. (2017-08-05) Human Gastrointestinal Metabolism of the Cistanches Herba Water Extract in Vitro: Elucidation of the Metabolic Profile Based on Comprehensive Metabolite Identification in Gastric Juice, Intestinal Juice, Human Intestinal Bacteria, and Intestinal Microsomes.. Journal of agricultural and food chemistry, 65 ((34)):(7447-7456). [PMID:28771352]
References
1.Li Guanglei, Chen Yao, Liu Fei, Bi Wenhua, Wang Chenxin, Lu Danfeng, Wen Dan. (2023) Portable visual and electrochemical detection of hydrogen peroxide release from living cells based on dual-functional Pt-Ni hydrogels. Microsystems & Nanoengineering, 9 (1):(1-10). [PMID:38033990][10.1038/s41378-023-00623-y]
2.Xu Daokun, Huang Haolun, Liu Zhen, Wang Yumei, Liu Qinan, Jiang Xing, Yang Jun, Ling Rui. (2023) Comprehensive characterization and detection of nut allergens in bakery foods using Q–TOF mass spectrometry and bioinformatics. Food Quality and Safety, (1):[PMID:11741838][10.1093/fqsafe/fyad061]
3.Jie Zhao, Shuaiqian Wang, Diandian Jiang, Yan Lu, Yu Chen, Yong Tang, Jie Tang, Zhenju Jiang, Hongbin Lin, Wei Dong. (2023) Unravelling the interaction between α-SOH and myofibrillar protein based on spectroscopy and molecular dynamics simulation. Food Chemistry-X, 20 (100986). [PMID:38144868][10.1016/j.fochx.2023.100986]
4.Zhe Chen, Yunxia Wang. (2023) A label- and enzyme-free fluorescence assay based on thioflavin T–induced G-quadruplexes for the detection of telomerase activity. JOURNAL OF CHEMICAL RESEARCH, [PMID:][10.1177/17475198221139085]
5.Pengfei Dong, Longyi Zhu, Jing Huang, Jujie Ren, Jianping Lei. (2019) Electrocatalysis of cerium metal-organic frameworks for ratiometric electrochemical detection of telomerase activity. BIOSENSORS & BIOELECTRONICS, 138 (111313). [PMID:31108380][10.1016/j.bios.2019.05.018]
6.Min Zhang, Chong-sheng Peng, Xiao-bo Li. (2017) Human intestine and liver microsomal metabolic differences between C19-diester and monoester diterpenoid alkaloids from the roots of Aconitum carmichaelii Debx.. TOXICOLOGY IN VITRO, 45 (318). [PMID:28919359][10.1016/j.tiv.2017.09.011]
7.Feifei Gong, Huaxin Yang, Wen Sun, Junzi Cao, Wanhui Liu. (2016) Development and validation of a micellar electrokinetic capillary chromatography method for the determination of goserelin and related substances. ELECTROPHORESIS, 37 (4):(623-629). [PMID:26635325][10.1002/elps.201500328]
8.Yang Li,Ying Peng,Mengyue Wang,Pengfei Tu,Xiaobo Li. (2017-08-05) Human Gastrointestinal Metabolism of the Cistanches Herba Water Extract in Vitro: Elucidation of the Metabolic Profile Based on Comprehensive Metabolite Identification in Gastric Juice, Intestinal Juice, Human Intestinal Bacteria, and Intestinal Microsomes.. Journal of agricultural and food chemistry, 65 ((34)):(7447-7456). [PMID:28771352]
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