Zwitterionic non-denaturing detergent that protects the native state of proteins, able to disrupt non-specific protein interactions and has less protein aggregation than non-ionic detergents. It is electrically neutral and easily removed by dialysis.
Storage Temp
Argon charged
Shipped In
Normal
Grade
AR
Note
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description
CHAPS is a non-denaturing, zwitterionic detergent derived from cholic acid. CHAPS is often used as a detergent in the solubilization and purification of membrane proteins for several advantageous reasons. CHAPS detergent is non-denaturing to membrane proteins, can solubilize proteins, disaggregate protein-protein interactions and is electrically neutral. CHAPS is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH 2 to 12. The critical micelle concentration of CHAPS is 6-10mM. A non-denaturing, zwitterionic detergent for membrane proteins
1.Li Guanglei, Chen Yao, Liu Fei, Bi Wenhua, Wang Chenxin, Lu Danfeng, Wen Dan. (2023) Portable visual and electrochemical detection of hydrogen peroxide release from living cells based on dual-functional Pt-Ni hydrogels. Microsystems & Nanoengineering, 9 (1):(1-10). [PMID:38033990][10.1038/s41378-023-00623-y]
2.Xu Daokun, Huang Haolun, Liu Zhen, Wang Yumei, Liu Qinan, Jiang Xing, Yang Jun, Ling Rui. (2023) Comprehensive characterization and detection of nut allergens in bakery foods using Q–TOF mass spectrometry and bioinformatics. Food Quality and Safety, (1):[PMID:11741838][10.1093/fqsafe/fyad061]
3.Jie Zhao, Shuaiqian Wang, Diandian Jiang, Yan Lu, Yu Chen, Yong Tang, Jie Tang, Zhenju Jiang, Hongbin Lin, Wei Dong. (2023) Unravelling the interaction between α-SOH and myofibrillar protein based on spectroscopy and molecular dynamics simulation. Food Chemistry-X, 20 (100986). [PMID:38144868][10.1016/j.fochx.2023.100986]
4.Zhe Chen, Yunxia Wang. (2023) A label- and enzyme-free fluorescence assay based on thioflavin T–induced G-quadruplexes for the detection of telomerase activity. JOURNAL OF CHEMICAL RESEARCH, [PMID:][10.1177/17475198221139085]
5.Pengfei Dong, Longyi Zhu, Jing Huang, Jujie Ren, Jianping Lei. (2019) Electrocatalysis of cerium metal-organic frameworks for ratiometric electrochemical detection of telomerase activity. BIOSENSORS & BIOELECTRONICS, 138 (111313). [PMID:31108380][10.1016/j.bios.2019.05.018]
6.Min Zhang, Chong-sheng Peng, Xiao-bo Li. (2017) Human intestine and liver microsomal metabolic differences between C19-diester and monoester diterpenoid alkaloids from the roots of Aconitum carmichaelii Debx.. TOXICOLOGY IN VITRO, 45 (318). [PMID:28919359][10.1016/j.tiv.2017.09.011]
7.Feifei Gong, Huaxin Yang, Wen Sun, Junzi Cao, Wanhui Liu. (2016) Development and validation of a micellar electrokinetic capillary chromatography method for the determination of goserelin and related substances. ELECTROPHORESIS, 37 (4):(623-629). [PMID:26635325][10.1002/elps.201500328]
8.Yang Li,Ying Peng,Mengyue Wang,Pengfei Tu,Xiaobo Li. (2017-08-05) Human Gastrointestinal Metabolism of the Cistanches Herba Water Extract in Vitro: Elucidation of the Metabolic Profile Based on Comprehensive Metabolite Identification in Gastric Juice, Intestinal Juice, Human Intestinal Bacteria, and Intestinal Microsomes.. Journal of agricultural and food chemistry, 65 ((34)):(7447-7456). [PMID:28771352]
References
1.Awada Z et al.. (2019) DNA methylome-wide alterations associated with estrogen receptor-dependent effects of bisphenols in breast cancer.. Clin Epigenetics, 11 (138). [PMID:31601247]
2.Li Guanglei, Chen Yao, Liu Fei, Bi Wenhua, Wang Chenxin, Lu Danfeng, Wen Dan. (2023) Portable visual and electrochemical detection of hydrogen peroxide release from living cells based on dual-functional Pt-Ni hydrogels. Microsystems & Nanoengineering, 9 (1):(1-10). [PMID:38033990][10.1038/s41378-023-00623-y]
3.Xu Daokun, Huang Haolun, Liu Zhen, Wang Yumei, Liu Qinan, Jiang Xing, Yang Jun, Ling Rui. (2023) Comprehensive characterization and detection of nut allergens in bakery foods using Q–TOF mass spectrometry and bioinformatics. Food Quality and Safety, (1):[PMID:11741838][10.1093/fqsafe/fyad061]
4.Jie Zhao, Shuaiqian Wang, Diandian Jiang, Yan Lu, Yu Chen, Yong Tang, Jie Tang, Zhenju Jiang, Hongbin Lin, Wei Dong. (2023) Unravelling the interaction between α-SOH and myofibrillar protein based on spectroscopy and molecular dynamics simulation. Food Chemistry-X, 20 (100986). [PMID:38144868][10.1016/j.fochx.2023.100986]
5.Zhe Chen, Yunxia Wang. (2023) A label- and enzyme-free fluorescence assay based on thioflavin T–induced G-quadruplexes for the detection of telomerase activity. JOURNAL OF CHEMICAL RESEARCH, [PMID:][10.1177/17475198221139085]
6.Pengfei Dong, Longyi Zhu, Jing Huang, Jujie Ren, Jianping Lei. (2019) Electrocatalysis of cerium metal-organic frameworks for ratiometric electrochemical detection of telomerase activity. BIOSENSORS & BIOELECTRONICS, 138 (111313). [PMID:31108380][10.1016/j.bios.2019.05.018]
7.Min Zhang, Chong-sheng Peng, Xiao-bo Li. (2017) Human intestine and liver microsomal metabolic differences between C19-diester and monoester diterpenoid alkaloids from the roots of Aconitum carmichaelii Debx.. TOXICOLOGY IN VITRO, 45 (318). [PMID:28919359][10.1016/j.tiv.2017.09.011]
8.Feifei Gong, Huaxin Yang, Wen Sun, Junzi Cao, Wanhui Liu. (2016) Development and validation of a micellar electrokinetic capillary chromatography method for the determination of goserelin and related substances. ELECTROPHORESIS, 37 (4):(623-629). [PMID:26635325][10.1002/elps.201500328]
9.Yang Li,Ying Peng,Mengyue Wang,Pengfei Tu,Xiaobo Li. (2017-08-05) Human Gastrointestinal Metabolism of the Cistanches Herba Water Extract in Vitro: Elucidation of the Metabolic Profile Based on Comprehensive Metabolite Identification in Gastric Juice, Intestinal Juice, Human Intestinal Bacteria, and Intestinal Microsomes.. Journal of agricultural and food chemistry, 65 ((34)):(7447-7456). [PMID:28771352]
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