After the protein sample has been spiked with Sampling Buffer, boiled at 95°C for 10 min, and centrifuged at 14,000, individual samples will be delaminated prior to sampling, what is the reason for this?
Protein samples boiled in buffer are blue in color and turn blue-violet after being left at -80°C. What is the reason for this? Does it affect the subsequent electrophoresis?
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