After the protein sample has been spiked with Sampling Buffer, boiled at 95°C for 10 min, and centrifuged at 14,000, individual samples will be delaminated prior to sampling, what is the reason for this?

After the protein sample has been spiked with Sampling Buffer, boiled at 95°C for 10 min, and centrifuged at 14,000, individual samples will be delaminated prior to sampling, what is the reason for this?

The Sample Buffer needs to be well mixed before use, and the sample and Sample Buffer need to be well mixed. Boiling and denaturing the sample after well mixing can avoid this situation.