The electrophoretic mobility shift assay (Electrophoretic Mobility Shift Assay, EMSA) is a classical method for studying protein–nucleic acid interactions. It relies on the observation that binding of a protein to DNA or RNA alters the complex’s net charge, conformation, and hydrodynamic ...
Nucleic acid research spans confirmation of DNA/RNA presence, sequence-level characterization, and mechanistic dissection of interactions between nucleic acids and proteins or other nucleic acids. The choice of experimental method determines evidence type, resolution, throughput, and ...
The electrophoretic mobility shift assay (EMSA), also known as the gel shift assay, is a classic in vitro technique used to detect DNA–protein or RNA–protein interactions under non-denaturing polyacrylamide gel electrophoresis (native PAGE) conditions.
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