Endoproteinase Lys-C: Mechanism, Applications, and Method-Control Considerations for Lysine-Specific Digestion

Endoproteinase Lys-C (Lys-C) is a lysine-specific serine endopeptidase that preferentially cleaves peptide bonds on the C-terminal side of lysine residues (Lys, K) under mildly alkaline conditions (typically pH 7.0–9.0).