Topic: High-performance liquid chromatography

Articles by Topic "High-performance liquid chromatography"

Knowledge Base
Technical articles

Porous Graphitic Carbon (PGC) Chromatography Columns: Creating New Selectivity for Challenging Separations through Molecular Shape and the π-Electron Cloud on the Graphitic Surface

In high-performance liquid chromatography (High Performance Liquid Chromatography, HPLC), octadecylsilane-bonded stationary phase (Octadecylsilane, C18) is the most commonly used reversed-phase stationary phase. The main separation basis of C18 is hydrophobic partitioning: the more hydrophobic a ...

Updated Jun 20268 min read ✓ Reviewed
Technical articles

Methods and Applications for the Determination of Amino Acid Content and Composition

Amino acid analysis is a fundamental technical component in biological sample analysis, nutritional evaluation, metabolism research, and fermentation process control. Its analytical targets include free amino acids, total amino acids after hydrolysis, specific single amino acids, and certain ...

Updated Apr 20268 min read ✓ Reviewed
Technical articles

Technical Comparison and Method Selection for Hydroxyproline Determination

Hydroxyproline determination is a classical quantitative technique in collagen-related research and is widely used in fibrosis assessment, tissue-remodeling studies, collagen-material analysis, and pharmacodynamic evaluation.

Updated Apr 20268 min read ✓ Reviewed
Technical articles

Analytical Basis, Methodological Systems, and Quality Control for Vitamin C Content Determination

Vitamin C content determination is not merely a matter of quantification, but rather a comprehensive analytical issue involving definition of the target analyte form, sample stabilization procedures, methodological selection, and the interpretive boundaries of analytical results. Because vitamin ...

Updated Mar 20268 min read ✓ Reviewed
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