Protoplasts are formed by the differentiation of protoplasm, specifically the cell membrane and intramembranous cytoplasm and other life-active organelles. In p
Under non-selective growth conditions, most E. coli/yeast shuttle vectors are lost at a frequency of about 1%, and one plasmid that is not easily isolated and r
A general method for cloning yeast genes by complementation using a putative mutation. The mutation is cdc101-1, which is both recessive and temperature sensiti
Because of the simplicity and sensitivity of the β-galactosidase assay, yeast genes are frequently labeled with the functional portion of the lacZ gene to indi
The meiotic products are released from the ascospores, ruptured by sonication, and spread directly on agar plates, and the spore colonies can be screened for th
Making anatomical needles requires patience and practical experience. The common practice is to make a long, thin glass filament, cut it into small segments, an
A standard light microscope is required for yeast tetrad analysis, and the microscope stage should be able to be moved along the x and y axes and the distance t
Diploid yeast cells induce meiosis and spore formation when they are starved of both nitrogen and carbon sources. At this point, their chromosomes replicate and
Microbial cell size, one of the morphological characteristics of microorganisms, is also one of the bases for taxonomic identification. Since the organism is ve
Ultraviolet sterilization is carried out with ultraviolet lamps, the wavelength of 200 ~ 300 nm of ultraviolet light have the ability to sterilize, of which 260
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