Protocols

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  1. Typing experiments with autofluorescence The typing methods described in this unit are needed when there are a large number of samples with multiple microsatellite markers to be typed. For example, a d
  2. Experiments on the preparation of chromosome smears in the mid-division of chorionic specimens The interstitial center of the villi includes a range of cellular components-fibroblasts, endothelial cells, and macrophages-as well as a large amount of interc
  3. High-throughput genotyping experiments using the TAQMAN assay This unit describes the application of 5'-nuclease allele specific analysis or TaqMan method for single nucleotide polymorphism (SNP) typing.
  4. Liposomal vectors for direct in vivo gene transfection experiments Many of the cationic liposomes used for liposomal transfection of cells in tissue cultures are able to be purchased. Composition optimization is highly dependen
  5. Experiments in the culture and preparation of samples of pregnancy products and other solid tissue sources for chromosome analysis In clinical practice, chromosomal studies require the use of solid tissues other than tumor tissue for one of two reasons: some patients can only obtain solid t
  6. Diagnosis of Fanconi anemia (congenital myelodysplasia)--dioxobutane (dibutyl epoxide) detection assay Fanconi anemia (FA) is an autosomal recessive syndrome characterized by progressive bone marrow failure and an increased risk of malignancies, particularly acut
  7. E. coli growth curve measurement experiment A certain amount of microorganisms, inoculated in a suitable fresh liquid medium, cultured at a suitable temperature, the logarithm of the number of bacteria as
  8. Experiments for the examination of culture characteristics of microorganisms Culture characteristics of microorganisms refer to the community morphology and growth exhibited by microorganisms cultured on media. The culture characteristic
  9. Ultraviolet sterilization test Ultraviolet sterilization is carried out with ultraviolet lamps, the wavelength of 200 ~ 300 nm of ultraviolet light have the ability to sterilize, of which 260
  10. High Pressure Steam Sterilization Experiment The autoclave experiment is applied to: sterilization. High-pressure steam sterilization is the items to be sterilized in a closed pressurized sterilizer pot, t
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