Loading Controls are antibodies that are used to normalize and quantify protein expression levels, usually against proteins encoded by "housekeeping genes" that are expressed at relatively constant levels in cells. The expression of housekeeping genes is relatively stable in different cell types, tissues and physiological states, and thus is often used as a reference standard in experiments to ensure the accuracy and comparability of experimental results.
< The Main Role of the Loading Controls >
I. Correction of Experimental Errors
II. Verify the Experimental Procedure
III. Frame of Reference
IV. Comparison of Protein Expression Between Samples
< Main Application Scenarios >
I. Western Blot（WB）
II. Immunohistochemistry (IHC)
III. Fluorescence-activated Cell Sorting(FACS)
IV. Enzyme-linked Immunosorbent Assay (ELISA)
V. Immunoprecipitation (IP)
< Aladdin Loading Controls >
I. High Specificity: Designed to minimize cross-reactivity and non-specific binding with clear localization and low background noise.
II. High Stability: Our loading controls provide robust, reproducible results that are consistent across a wide range of experimental conditions.
III. Strong Reliability: Our loading controls have undergone rigorous application validation to ensure specificity and reliability.
IV. Rich Variety: We provide antibodies targeting a variety of internal reference proteins, including β-actin, α/β/γ-Tubulin, Histone H3, Lamin B1, PCNA, TBP, GAPDH, COX Ⅳ, Cyclophilin A/B, etc., to meet the needs of a variety of experiments.
Loading controls are indispensable stabilizing references in experiments and play a crucial role in both cell biology and molecular biology. Aladdin Scientific is committed to providing you with high-quality research reagents to help you in your scientific exploration journey.
Feel free to visit our website for more details and to browse our comprehensive Aladdin Loading Controls.