What is the reason why the protein after denaturation by adding buffer tends to be difficult to sink and float up easily when spotting? What should I do to solve this problem?
What is the reason why the protein after denaturation by adding buffer tends to be difficult to sink and float up easily when spotting? What should I do to solve this problem?
posted this at Jun 9, 2025 Updated at Aug 27, 2024
What is the reason why the protein after denaturation by adding buffer tends to be difficult to sink and float up easily when spotting? What should I do to solve this problem?
It may be because the glycerol in the buffer is not completely mixed, it is recommended that the buffer be completely dissolved at room temperature or at no more than 37℃ before use.
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