Articles by Topic "protein"

1. How Biotinylated Molecules are Purified with Streptavidin Beads
   posted this at Nov 27, 2025

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   Streptavidin is a bacterial protein that binds the small molecule biotin with exceptionally high affinity. The streptavidin–biotin pair represents one of the strongest noncovalent interactions known. Streptavidin agarose beads take advantage of this interaction as a solid-phase platform to ...
2. Common Protein Gel Staining Methods
   posted this at Nov 20, 2025

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   After proteins are separated by SDS-PAGE or related gels, staining is used for visualization, rough quantification, and purity assessment.
3. Protein Tris-Acetate Electrophoresis System Standard Operating Procedure
   posted this at Nov 7, 2025

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   Protein Tris-Acetate Electrophoresis System Standard Operating Procedure
4. Protein Molecular Weight Standards
   posted this at Nov 7, 2025

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5. Principles of Enzymology and Its Biotechnological Applications
   posted this at Sep 12, 2025

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   Enzymes are highly efficient and specific biological catalysts, most of which are proteins. They can significantly accelerate the rate of biochemical reactions without altering the reaction equilibrium.
6. What solution should be used to dissolve Proteinase K when used? What is the common working concentration? At what degree does it need to be stored?
   posted this at Jun 9, 2025

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   What solution should be used to dissolve Proteinase K when used? What is the common working concentration? At what degree does it need to be stored?
7. Does Proteinase K powder need to be protected from light when storing and weighing?
   posted this at Jun 9, 2025

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   Does Proteinase K powder need to be protected from light when storing and weighing?
8. Does paraformaldehyde fixation disrupt cell membranes?
   posted this at Jun 9, 2025

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   Does paraformaldehyde fixation disrupt cell membranes?
9. Can ECL be used in ELISA?
   posted this at Jun 9, 2025

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   Can ECL be used in ELISA?
10. After lysing the cells first with the lysate, add 5x upsampling buffer in a 4:1 volume, then water bath denaturation is it better to use a 100 degree water bath or a 95 degree water bath?
    posted this at Jun 9, 2025

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    After lysing the cells first with the lysate, add 5x upsampling buffer in a 4:1 volume, then water bath denaturation is it better to use a 100 degree water bath or a 95 degree water bath?