After lysing the cells first with the lysate, add 5x upsampling buffer in a 4:1 volume, then water bath denaturation is it better to use a 100 degree water bath or a 95 degree water bath?
After lysing the cells first with the lysate, add 5x upsampling buffer in a 4:1 volume, then water bath denaturation is it better to use a 100 degree water bath or a 95 degree water bath?
posted this at Jun 9, 2025 Updated at Aug 27, 2024
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After lysing the cells first with the lysate, add 5x upsampling buffer in a 4:1 volume, then water bath denaturation is it better to use a 100 degree water bath or a 95 degree water bath?
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