Irreversible serine protease inhibitor. Inhibition constants are similar to those of PMSF and DFP. AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin kallikrein and thrombin. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, impr
Storage Temp
Store at -20°C,Argon charged
Shipped In
Ice chest + Ice pads
Product Description
4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) has been used: as a protease inhibitor in plasma samples to prevent acylated ghrelin (AG) degradation as a snake venom serine proteinase (SVSP) inhibitor in SVSP inhibition or AEBSF assay to test the involvement of SVSPs on the fibrinogen-clotting activity as a component in NP-40 lysis buffer to resuspend cell pellets for preparing cell lysates Recommended concentrations for use are 0.1-1 mM.
1.Chenglin Li, Sifan Li, Xinsheng Li, Tao Yuan, Jialei Xu, Xixin Gu, Jianli Hua. (2023) A Turn-On Lipid Droplet-Targeted Near-Infrared Fluorescent Probe with a Large Stokes Shift for Detection of Intracellular Carboxylesterases and Cell Viability Imaging. MOLECULES, 28 (5):(2317). [PMID:36903562][10.3390/molecules28052317]
2.Yi-Xuan Fu, Wu-Yingzheng Guo, Nan Wang, Yi-Jie Dai, Zi-Ye Zhang, Xin-Lin Sun, Wen-Chao Yang, Guang-Fu Yang. (2022) Diagnosis of Bacterial Plant Diseases via a Nitroreductase-Activated Fluorescent Sensor. ANALYTICAL CHEMISTRY, 94 (50):(17692–17699). [PMID:36469707][10.1021/acs.analchem.2c04614]
3.Jingmin Zhang, Youmei Peng, Ying Li, Ning Wang, Yuna Chai, Chongzhen Qin, Xinru Wang, Suna Liu, Yubing Zhou, Xiaojian Zhang, Wenda Zhang. (2022) Development of a near-infrared fluorescent probe with large Stokes shift for carboxylesterases detection and its application in living systems. DYES AND PIGMENTS, 198 (109993). [PMID:][10.1016/j.dyepig.2021.109993]
4.Yu Zhu Yang, Zi Yi Xu, Nian Bing Li, Hong Qun Luo. (2021) Ultrasensitive fluorescent probe for visual biosensing of esterase activity in living cells and its imaging application. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 262 (120094). [PMID:34175764][10.1016/j.saa.2021.120094]
5.Shuli Man, Miao Li, Jin Zhou, Haiyue Wang, Jinyan Zhang, Long Ma. (2019) Polyethyleneimine coated Fe3O4 magnetic nanoparticles induce autophagy, NF-κB and TGF-β signaling pathway activation in HeLa cervical carcinoma cells via reactive oxygen species generation. Biomaterials Science, 8 (1):(201-211). [PMID:31664285][10.1039/C9BM01563A]
6.Hui Zhou, Jinbao Tang, Jie Zhang, Bochao Chen, Jianfei Kan, Weifen Zhang, Jin Zhou, Huimin Ma. (2019) A red lysosome-targeted fluorescent probe for carboxylesterase detection and bioimaging. Journal of Materials Chemistry B, 7 (18):(2989-2996). [PMID:][10.1039/C9TB00310J]
7.Juan Wang, Mengmeng Song, Chengguo Hu, Kangbing Wu. (2018) Portable, Self-Powered, and Light-Addressable Photoelectrochemical Sensing Platforms Using pH Meter Readouts for High-Throughput Screening of Thrombin Inhibitor Drugs. ANALYTICAL CHEMISTRY, 90 (15):(9366–9373). [PMID:29998727][10.1021/acs.analchem.8b01979]
References
1.Marinea M et al.. (2021) Soy Protein Pressed Gels: Gelation Mechanism Affects the In Vitro Proteolysis and Bioaccessibility of Added Phenolic Acids.. Foods, 10 [PMID:33450925]
2.Chenglin Li, Sifan Li, Xinsheng Li, Tao Yuan, Jialei Xu, Xixin Gu, Jianli Hua. (2023) A Turn-On Lipid Droplet-Targeted Near-Infrared Fluorescent Probe with a Large Stokes Shift for Detection of Intracellular Carboxylesterases and Cell Viability Imaging. MOLECULES, 28 (5):(2317). [PMID:36903562][10.3390/molecules28052317]
3.Yi-Xuan Fu, Wu-Yingzheng Guo, Nan Wang, Yi-Jie Dai, Zi-Ye Zhang, Xin-Lin Sun, Wen-Chao Yang, Guang-Fu Yang. (2022) Diagnosis of Bacterial Plant Diseases via a Nitroreductase-Activated Fluorescent Sensor. ANALYTICAL CHEMISTRY, 94 (50):(17692–17699). [PMID:36469707][10.1021/acs.analchem.2c04614]
4.Jingmin Zhang, Youmei Peng, Ying Li, Ning Wang, Yuna Chai, Chongzhen Qin, Xinru Wang, Suna Liu, Yubing Zhou, Xiaojian Zhang, Wenda Zhang. (2022) Development of a near-infrared fluorescent probe with large Stokes shift for carboxylesterases detection and its application in living systems. DYES AND PIGMENTS, 198 (109993). [PMID:][10.1016/j.dyepig.2021.109993]
5.Yu Zhu Yang, Zi Yi Xu, Nian Bing Li, Hong Qun Luo. (2021) Ultrasensitive fluorescent probe for visual biosensing of esterase activity in living cells and its imaging application. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 262 (120094). [PMID:34175764][10.1016/j.saa.2021.120094]
6.Shuli Man, Miao Li, Jin Zhou, Haiyue Wang, Jinyan Zhang, Long Ma. (2019) Polyethyleneimine coated Fe3O4 magnetic nanoparticles induce autophagy, NF-κB and TGF-β signaling pathway activation in HeLa cervical carcinoma cells via reactive oxygen species generation. Biomaterials Science, 8 (1):(201-211). [PMID:31664285][10.1039/C9BM01563A]
7.Hui Zhou, Jinbao Tang, Jie Zhang, Bochao Chen, Jianfei Kan, Weifen Zhang, Jin Zhou, Huimin Ma. (2019) A red lysosome-targeted fluorescent probe for carboxylesterase detection and bioimaging. Journal of Materials Chemistry B, 7 (18):(2989-2996). [PMID:][10.1039/C9TB00310J]
8.Juan Wang, Mengmeng Song, Chengguo Hu, Kangbing Wu. (2018) Portable, Self-Powered, and Light-Addressable Photoelectrochemical Sensing Platforms Using pH Meter Readouts for High-Throughput Screening of Thrombin Inhibitor Drugs. ANALYTICAL CHEMISTRY, 90 (15):(9366–9373). [PMID:29998727][10.1021/acs.analchem.8b01979]
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A2503788
