Peroxy Orange 1 (PO1) - BioReagent,Biological Stain,for fluorescence analysis,for microscopy,≥98% , CAS No.1199576-10-7

CAS: 1199576-10-7 Cat. No.: P287730 Peso molecular: 521.41 PubChem CID: 44546671

GRADE & PURITY BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥98%

Synonyms

PO1 | 2',3',6',7'-Tetrahydro-12'-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-spiro[isobenzofuran-1(3H),9'-[1H,5H,9H]xantheno[2,3,4-ij]quinolizin]-3-one | 2-(3,6-Diacetoxy-9H-xanthen-9-yl)benzoic acid | 2-[3,6-bis(acetyloxy)-9H-xanthen-9-yl]benzoic acid

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Cell Staining, Fluorescence Analysis

Descripción general

Peroxy Orange 1 (PO1) is a novel hydrogen peroxide (H₂O₂)-specific probe, which can be combined with APF, a green fluorescent highly reactive oxygen species (hROS) probe. Peroxy Orange 1 also serves as a live-cell dye.As a highly sensitive fluorescent probe for H₂O₂, Peroxy Orange 1 emits orange fluorescence with an excitation wavelength of 543 nm and an emission wavelength of 565 nm.Peroxy Orange 1 contains boronate ester groups, which can be oxidized into phenol groups by H₂O₂ to produce bright orange fluorescence.This boronate-phenol transformation mechanism does not react with other reactive oxygen species (ROS) such as superoxide anions and NO, thus guaranteeing the high selectivity of Peroxy Orange 1 toward H₂O₂.Peroxy Orange 1 exhibits excellent cell permeability, enabling the detection of intracellular H₂O₂ in live cells with negligible cytotoxicity.It is compatible with conventional green fluorescent probes including GFP and APF, and suitable for multicolor fluorescence imaging assays.

Specifications

Sinónimos

PO1 | 2', 3', 6', 7'-Tetrahydro-12'-(4, 4, 5, 5-tetramethyl-1, 3, 2-dioxaborolan-2-yl)-spiro[isobenzofuran-1(3H), 9'-[1H, 5H, 9H]xantheno[2, 3, 4-ij]quinolizin]-3-one | 2-(3, 6-Diacetoxy-9H-xanthen-9-yl)benzoic acid | 2-[3, 6-bis(acetyloxy)-9H-xanthen-9-yl]benzoic acid

Especificaciones y pureza

BioReagent, Biological Stain, for fluorescence analysis, for microscopy, ≥98%

Mecanismos bioquímicos y fisiológicos

Fluorescent probe for imaging hydrogen peroxide (H2O2); cell permeable. Displays an orange intracellular fluorescence in response to H2O2signals produced in RAW 264.7 macrophages and A431 cells during immune response and growth factor stimulation, respect

Estabilidad y almacenamiento

Store at -20℃ long term (24 months). Store in the dark.

Condiciones de almacenamiento de almacenamiento

Protected from light, Store at -20°C

Enviado en

Ice chest + Ice pads

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Grado

Biological Stain, BioReagent, for Fluorescence analysis, for Microscopy

Pureza

≥98%

Nombres e identificadores

• Sonrisas canónicas: B1(OC(C(O1)(C)C)(C)C)C2=CC3=C(C=C2)C4(C5=CC=CC=C5C(=O)O4)C6=C(O3)C7=C8C(=C6)CCCN8CCC7
• IUPAC Name: 6'-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)spiro[2-benzofuran-3,10'-3-oxa-17-azapentacyclo[11.7.1.02,11.04,9.017,21]henicosa-1(21),2(11),4(9),5,7,12-hexaene]-1-one
• InChIKey: KNTNXZHHCPNNGB-UHFFFAOYSA-N
• INCHI: 1S/C32H32BNO5/c1-30(2)31(3,4)39-33(38-30)20-13-14-24-26(18-20)36-28-22-11-8-16-34-15-7-9-19(27(22)34)17-25(28)32(24)23-12-6-5-10-21(23)29(35)37-32/h5-6,10,12-14,17-18H,7-9,11,15-16H2,1-4H3
• Isómeros SMILES: B1(OC(C(O1)(C)C)(C)C)C2=CC3=C(C=C2)C4(C5=CC=CC=C5C(=O)O4)C6=C(O3)C7=C8C(=C6)CCCN8CCC7
• PubChem CID: 44546671
• Peso molecular: 521.41

Propiedades químicas y físicas

• Solubilidad: Solubility in DMSO: 5.21 mg/mL (9.99 mM; dissolved with ultrasonication and heating; Hygroscopic DMSO significantly affects product solubility. Freshly opened DMSO is recommended.)
• Sensibilidad: Light-sensitive
• Peso molecular: 521.400 g/mol
• Hydrogen Bond Donor Count: 0
• Hydrogen Bond Acceptor Count: 6
• Rotatable Bond Count: 1
• Exact Mass: 521.237 Da
• Monoisotopic Mass: 521.237 Da
• Topological Polar Surface Area: 57.200 Ų
• Heavy Atom Count: 39
• Formal Charge: 0
• Complexity: 987.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 1
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

XLogP3

Referencias

1. Zhou Liu, Lina Xie, Kangqiang Qiu, Xinxing Liao, Thomas W. Rees, Zizhuo Zhao, Liangnian Ji, Hui Chao.  (2020)  An Ultrasmall RuO2 Nanozyme Exhibiting Multienzyme-like Activity for the Prevention of Acute Kidney Injury.  ACS Applied Materials & Interfaces,      [PMID:32628016] [10.1021/acsami.0c07886]