β-Galactosidase (β-galactosidase, EC 3.2.1.23) is a glycoside hydrolase family that catalyzes hydrolysis of β-D-galactosidic bonds. It can cleave lactose and related substrates into monosaccharides and, under certain conditions, carry out transgalactosylation to generate products such as ...
Blue-white screening of bacterial colonies is a popular and effective molecular biology tool often used to detect recombinant bacteria in cloning experiments. Central to this technique is the enzymatic activity of β-galactosidase, a tetrameric enzyme encoded by the lacZ α gene in E. coli that ...
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