Capture assays for interacting proteins are subjected to two sequential bulk yeast plate screening processes. Saccharomyces cerevisiae contains the lexA fusion
It is of interest to identify phosphorylated amino acid residues in proteins. Phosphorylation occurs at serine, threonine, and methionine in proteins, and the i
Protein is the material basis of life, without which there is no life. It is therefore a substance that is closely associated with life and with all forms of vi
Since host protein synthesis is essentially terminated when recombinant proteins are expressed, in vivo metabolic labeling is a sensitive method for detecting r
A gene fusion expression system using the E. coli trxA gene product-thioredoxin as a fusion partner is particularly suitable for the expression of high yield so
The pGEX vectors can be used to express and purify peptides (including short peptides) used as immunogens and biochemical and biological reagents, or to constru
A probe is a small fragment of single-stranded DNA or RNA (approximately 20 to 500 bp) that is used to detect a nucleic acid sequence complementary to it. Doubl
A technique for selecting a target from a population based on the principle of antigen-antibody interaction. For example, antibodies are used to detect proteins
Recovery test is a type of "control test". When the analyzed sample is complex and not completely clear, a known amount of the measured component is added to th
The affinity molecules with special structure are made into solid-phase adsorbent and placed in the chromatography column, when the protein mixture to be separa
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