In firefly luciferase reporter systems, signal quality is jointly determined by substrate accessibility, effective substrate concentration, reaction kinetics, and optical acquisition parameters. Substrate identity alone is rarely the only determinant; without control systems and parameter ...
Luciferase, as a core tool for bioluminescence detection, has been used for decades yet continues to inspire new methods and applications.Recent studies from both biomolecular engineering and optical imaging engineering point toward a common goal: to simultaneously and distinguishably observe ...
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