2-Mercaptoethylamine•HCl (2-MEA) is a mild reducing agent suitable for selectively cleaving hinge-region disulfide bonds of IgG heavy chains. Several popular methods for conjugation or immobilization of antibodies involve covalent attachment specifically through sulfhydryl (−SH) groups.
If I need to label an antibody with SMCC-activated R-phycoerythrinand then use the antibody coupled with phycoerythrin as an ELISA, what other reagents are needed? Is there a specific procedure?
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