RNase Free Grade
RNase Free Grade: Overview
RNase Free grade refers to reagents, consumables, and environments that are free of ribonuclease (RNase) contamination. RNases are enzymes that degrade RNA, and they are ubiquitous—found on human skin, in dust, and on laboratory surfaces. Because RNA is fragile and highly susceptible to degradation, any trace of RNase can ruin RNA samples, leading to inaccurate experimental results.
Applications of RNase Free Reagents
RNase Free reagents are critical for any procedure involving RNA extraction, purification, analysis, or manipulation, including:
· RNA isolation (e.g., from tissue or cells)
· RT-PCR / qRT-PCR (reverse transcription polymerase chain reaction)
· Northern blotting
· RNA-Seq library preparation
· In vitro transcription
· RNA interference (RNAi) studies
Common RNase Free Reagents and Tools
Here are several types of biological reagents and materials that are available in RNase Free grade:
Category | Examples |
Water & Buffers | RNase Free water, DEPC-treated water, PBS (RNase Free), Tris buffers |
Enzymes | RNase inhibitors, DNase I (RNase Free), reverse transcriptases |
Chemicals | Phenol-chloroform mixtures (RNase Free), guanidinium thiocyanate solutions |
Plasticware | RNase Free pipette tips, tubes, microcentrifuge tubes |
Kits | RNA extraction kits, cDNA synthesis kits (certified RNase Free) |
Surfactants & Detergents | RNase Free Triton X-100, Tween-20 |
How RNase Free Grade is Achieved
Manufacturers ensure RNase Free grade through:
1. Chemical treatment (e.g., DEPC treatment)
2. Autoclaving to inactivate RNase enzymes
3. Ultrafiltration during purification of reagents
4. Sterile packaging in RNase Free environments
5. RNase activity testing to validate absence of contamination
Best Practices for Working RNase Free
Even when using RNase Free reagents, handling protocols are essential:
· Wear gloves and change frequently
· Use dedicated RNase Free pipettes and tips
· Clean work areas with RNase-decontamination solutions
· Avoid talking or breathing directly over open tubes
View all RNase Free products