Experiments on the effect of hemolysis and jaundice on the determination of enzyme activity
Summary
To examine the accuracy of enzyme activity measurement in the presence of interfering substances, this experiment is from the Mudanjiang Medical College undergraduate 5-year laboratory guide for laboratory tests.
Operation method
Experiments on the effect of hemolysis and jaundice on the determination of enzyme activity
Principle
L-alanine + α-ketoglutarate ALT pyruvate + L-glutamate pyruvate + NADH + H+ → L-lactate + NAD+
Materials and Instruments
Jaundice Serum Move 1. Dilute the reagent. For more product details, please visit Aladdin Scientific website.
ALT/GPT reagent
Semi-automatic biochemical analyzer Test tubes Sampler
2、Pre-temperature up to 37 degrees.
3、Select the measurement program．
4, test:
5. Analysis of results:
Categories: Protocols