Molecular exclusion chromatography, also known as gel chromatography, is the use of different molecular sizes of the separated substances lead to different degr
It is of interest to identify phosphorylated amino acid residues in proteins. Phosphorylation occurs at serine, threonine, and methionine in proteins, and the i
A gene fusion expression system using the E. coli trxA gene product-thioredoxin as a fusion partner is particularly suitable for the expression of high yield so
The pGEX vectors can be used to express and purify peptides (including short peptides) used as immunogens and biochemical and biological reagents, or to constru
A probe is a small fragment of single-stranded DNA or RNA (approximately 20 to 500 bp) that is used to detect a nucleic acid sequence complementary to it. Doubl
Capture assays for interacting proteins are subjected to two sequential bulk yeast plate screening processes. Saccharomyces cerevisiae contains the lexA fusion
The first step in capturing interacting proteins is to construct a decoy protein, which is a fusion of LexA to the target protein. A series of control experimen
Metallothionein is a short cysteine-rich peptide produced by microorganisms and plants as a metal-binding protein with a high affinity for a wide range of heavy
Immunofluorescence localization of nuclear proteins can be applied to (1) localize intracellular distribution of nuclear proteins and (2) viral studies.
A technique for selecting a target from a population based on the principle of antigen-antibody interaction. For example, antibodies are used to detect proteins
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