Articles by Topic "ChIP"

1. A Review of Ten Commonly Used Techniques for Detection, Quantification, and Interaction Analysis in Nucleic Acid Research
   posted this at Jan 21, 2026

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   Nucleic acid research spans confirmation of DNA/RNA presence, sequence-level characterization, and mechanistic dissection of interactions between nucleic acids and proteins or other nucleic acids. The choice of experimental method determines evidence type, resolution, throughput, and ...
2. Dual Cross-linking ChIP Protocol
   posted this at Jun 9, 2025

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   ChIP is a powerful tool that uses isolated chromatin and antibodies against the target antigen to determine whether the target binds to a specific DNA sequence or to map its distribution across the genome (ChIP-seq).
3. CUT&RUN-seq protocol
   posted this at Jun 9, 2025

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   Targets and Release Using Nuclease (CUT&RUN) experimental technology is an emerging chromatin analysis method for studying protein-DNA interactions. It uses antibodies to specifically label target proteins, and then uses nucleases bound to antibodies (such as pAG-MNase) to cut DNA fragments that ...
4. Chromatin preparation from tissues for chromatin immunoprecipitation (ChIP)
   posted this at Jun 9, 2025

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   This protocol describes how chromatin is prepared from tissue, which can subsequently be used for chromatin immunoprecipitation (ChIP).
5. Cross-linking ChIP-Seq (X-ChIP-Seq) protocol
   posted this at Jun 9, 2025

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   Detailed procedure and tips for cross-linking ChIP using ChIP-seq and ChIP-qPCR methods.