Tandem mass spectrometry is an effective technique for peptide characterization based on the fragmentation pattern of the peptide, also known as MS/MS scanning.
MALDI-TOF peptide mass fingerprinting (PMF) is a faster and more convenient method for identifying proteins. The genomes studied have been sequenced and annotat
Immunoblotting using polyvinylidene difluoride (PVDF ) membranes is the most commonly used method for Edman sequencing. Protein samples are transferred from a 2
We provide background on multivariate analysis of proteomics data and also provide methods for data transformation of different data packages. This technique ca
Two-dimensional electrophoresis, as an important experimental tool for comparative proteomics studies, has been applied to conveniently reflect the differences
Staining of biphasic gel electrophoresis is a crucial step in comparative proteomics studies regarding the number of proteins to be measured, the accurate quant
Seeds may contain different components, like starch and polysaccharides, which can severely reduce the amount of protein extracted. The proteins in cereal plant
In this chapter we describe an efficient method suitable for whole protein extraction from various plant tissues. The method utilizes trichloroacetic acid and a
Another feasible method for protein extraction, besides the classical trichloroacetic acid (TCA ) /acetone precipitation method, is phenol extraction, which can
With the development of transgenic technology systems, the transfer of heterologous genes into the plant genome has become possible, which provides a new avenue
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