In Western Europe, for transgenic crops to be approved for commercial production, proof of their substantial equivalence to conventionally bred crops needs to b
Over the past 30 years, fluorescent chromosome analysis techniques have facilitated the understanding of genome construction and deepened knowledge of the organ
The study of insertion patterns and loci of exogenous genes in transgenic plants greatly contributes to the understanding of the mechanisms of integration, expr
Oat, a temperate cereal crop widely grown worldwide, lacks tolerance to osmotic stress caused by drought and/or high salt. We have developed a genotype-independ
Agrobacterium-mediated transformation of barley has been widely utilized over the last decade and it offers advantages over gene gun-mediated transgenic techniq
Microparticle bombardment, also known as the gene gun method, has been widely used in the genetic transformation of cereal crops. This method brings DNA directl
This chapter details a reproducible method of Agrobacterium-mediated transformation of spring wheat by direct inoculation of Agrobacterium sap into immature see
This chapter describes a method similar to the Arabidopsis inflorescence imbibition method for the transformation of wheat floral organs. Instead of relying on
Nitrate reductase (NR) is a key enzyme in the process of nitrate assimilation, which plays an important role in plant growth and development, and the determinat
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