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  1. Evaluation of lymphocyte-mediated cytotoxicity using flow cytometry Cytotoxicity of lymphocytes, is the killing of target cells by the release of particles containing perforin and granzyme and/or through the interaction of Fas-F
  2. Multi-parameter data acquisition and analysis experiments on leukocytes Several software packages are now available for flow cytometry data analysis. Although each one has a different approach in processing the data, the basic strat
  3. Preparation of monoclonal antibodies Hybridoma cells are formed by fusing an immune animal B lymphocyte that produces a single antibody with myeloma cells by cell fusion techniques (e.g., PEG-induc
  4. Isolation of peripheral blood single nucleated cells Immune cell isolation techniques are developing in the direction of more and more "fine", immune cell subpopulation and even micro-immune cell isolation and ide
  5. In vivo activation of macrophages Macrophages are important natural immune cells in vivo, and in vitro detection of their function does not fully reflect their in vivo behavior, while detection
  6. Induction and isolation of human blood mononuclear macrophages Macrophages (MΦ) are mature cells formed by the migration of blood monocytes into tissues, and are widely distributed in the spleen, liver, abdominal cavity, n
  7. Isolation and purification of human metaphase immune cells The metaphase immune cell population is the biological basis of maternal-fetal immune tolerance, and its composition is extremely special, mainly composed of sp
  8. CFSE assay for human peripheral blood T cell expansion in vitro CFSE detection of human peripheral blood T cell expansion in vitro refers to the use of CFSE to fluorescently label live cells, which can be used for the detect
  9. Measurement of immune cell phagocytosis The body has phagocytosis (phagocytosis) function of the cells called phagocytes, including monocyte macrophages and neutrophils two categories. Phagocytes phag
  10. Antibody potency assay Antibody potency assay is used to test the potency of immune serum. Currently there are three main methods used to determine antibody potency: tube agglutinatio
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