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  1. Immunogold labeling of tissue sections Immunogold labelling technique is a new type of immunolabeling technique applied to antigen-antibody reaction using colloidal gold as tracer. Colloidal gold is
  2. Separation of T cells and B cells by magnetic bead method This unit describes the separation of T cells, B cells, and T cell subpopulations by magnetic separation techniques. Author: J.E. Collier et al, Translator: Xui
  3. Cloning, Expression and Modification of Antibody Variable Regions (V Regions) PCR-based cloning schemes utilize conserved sequences in the framework and pilot regions of the variable region, and although primers can be synthesized with co
  4. Hydrolysis of immunoglobulin G (IgG) fragments When a new antibody needs to be fragmented, it is often necessary to perform a touch-and-go test. When choosing the reaction time, it is preferable to keep part
  5. Human α2a, α2b Interferon Assay An indispensable part of the production process of recombinant human α2a/α2b interferon is the quality control of the product, and the bioactivity o
  6. Interleukin 8 (IL- 8) Assay Il-8 is a polypeptide with a molecular weight of 8-10 kD, which has chemotactic effects on neutrophils, T lymphocytes, and alkaliphilic granulocytes, and activa
  7. Soluble Interleukin 2 Receptor Assay The level of concentration of soluble interleukin 2 receptor SIL-2R is closely related to many diseases, such as adult T-lymphoblastic leukemia AIDS, B-cell slo
  8. In situ PCR amplification of DNA and RNA target sequences In situ PCR, a technique established by Hasse in 1990, involves performing PCR reactions in tissue cells. It combines the advantages of in situ hybridization wi
  9. IL-10 biological activity assay In the presence of mouse IL-4 (Mil-4), IL-10 stimulated proliferation of the D36 mouse mast cell line. In contrast, single mIL4 or hIL-10 had only very low prol
  10. IL-8 Biological Activity Assay There are activating and chemotactic effects on neutrophils in IL-8, and the biological activity of IL-8, i.e., chemotactic activity on neutrophils, can be dete
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