This chapter describes how to prepare and use cDNA macroarrays to detect the effects of environmental toxins on gene expression, and describes the materials and
If the previous step in the series of analytical techniques for gene expression, SAGE cDNA, was stored at -20°C, then this experiment was thawed slowly on ice.
The T4 phage polynucleotide kinase-catalyzed exchange reaction (Van de Sande et al. 1973; Berkner and Folk 1977) is a rapid method for labeling 5' end DNA. Unli
In addition to cDNA microarrays, cDNA macroarrays based on nylon membrane supports are another widely used method for collecting large-scale gene expression dat
Specific antigen-induced cytotoxic T cell function assay is mainly applied to (1) the determination of immune cell function (2) the diagnosis of clinical autoim
Measurement of neutrophil phagocytosis can be used to (1) detect phagocytosis in the body and (2) diagnose diseases associated with phagocyte dysfunction.
With the wide application of enzyme-linked immunoassay (ELISA) technology in the field of medicine and biology, new breakthroughs have been made in the study of
When an antibody (or antigen) labeled with a radioactive element is precipitated with the corresponding antigen (or antibody), the precipitation line is confirm
Human umbilical vein endothelial cells (HUVEC) can be used (1) as a model cell for in vitro experiments and (2) to study the biological properties of vascular e
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