Protocols

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  1. M13 Cloning of phage vectors Although there is theoretically no limit to the number of fragments of exogenous DNA that can be carried by a recombinant M13 phage, in practice there is a limi
  2. Recombinant M13 phage cloning analysis In this protocol, when the exogenous DNA is larger than 200~300 nucleotides, it can be identified by gel electrophoresis analysis of the single-stranded DNA rel
  3. Construction of genomic DNA libraries using mucoid vectors The steps for constructing genomic DNA libraries in λ phage are essentially the same as in mucosal vectors. In both systems, eukaryotic DNA is ligated to vecto
  4. Preparation of single-stranded DNA using phage vectors Phage plasmids skillfully combine the characteristics of plasmids and filamentous phages. In addition to the basic features, these plasmids are usually high cop
  5. Tumor protein immunopreventive assay Immunological control refers to the application of various types of biological or non-biological biological agents to establish, enhance or inhibit the immune f
  6. Enzyme immunoelectrophoresis Enzyme immunoelectrophoresis can be applied to the analysis and identification of enzyme proteins.
  7. Insulin assay experiment Radioimmunoassay of insulin can be used for (1) immunolabeling detection techniques using radioisotopes as tracers, and (2) competitive binding of isotope-label
  8. Estradiol Detection Laboratory Estradiol (17β-Estradiol) is an important component of natural estrogen, and the detection of estradiol can be used for (1) the determination of hormone additi
  9. Polyclonal Antibody Preparation Experiment Antigens are usually composed of multiple antigenic determinants, and the antibody produced by a B-lymphocyte receiving an antigen stimulated by one antigenic d
  10. Polyclonal antibody purification assay The octanoic acid extraction method can be used to isolate and extract Ig from human, rabbit and mouse serum and McAb from hybridoma-induced ascites and culture
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