Nucleic acid probe molecular hybridization refers to the two nucleic acid single strands with certain homology can be formed under certain conditions according
The cDNA library construction can be: (1) used to isolate full-length genes for gene function studies; (2) screen target genes and use them directly for express
The construction and screening of DNA libraries can be used to: (1) cut the entire genomic DNA of an organism into DNA fragments of a certain length by restrict
M13 phage is a filamentous phage that does not lyse bacterial cells after infection, but only utilizes intracellular material to complete its own proliferation,
The basic principle of plasmid DNA extraction by alkaline lysis is that solution I suspends the cells and EDTA can chelate with Ca2+ and Mg2+ to inhibit the act
Spot and narrow line blotting experiments that can be used to detect the relative abundance of target sequences in blotted 1DNA products. The main applications
Nucleic acid electrophoresis is an important tool for conducting nucleic acid research and can be used for techniques such as (1) nucleic acid probing; (2) nucl
Luciferase plasmids were constructed and then transfected into cells to detect alterations in reporter gene expression by comparing overexpression or interferen
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