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  1. Ligation, transformation and screening of recombinant plasmids Recombinant plasmid ligation, transformation and screening are mainly used to obtain recombinants containing the target gene linkage.
  2. Plate-laying and transfer experiments for stickies and plasmid libraries The library should be amplified as soon as possible once it is packaged; it can greatly increase the copy number of the library, but there may be some potential
  3. Plate laying and transfer experiments of phage libraries The library should be amplified as soon as possible once it is packaged; it can greatly increase the copy number of the library, but there may be some potential
  4. Amplification experiments of phage libraries The library should be amplified as soon as possible once it is packaged; it can greatly increase the copy number of the library, but there may be some potential
  5. Pulsed field gel electrophoresis experiment Pulsed-field gel electrophoresis, which alternates periodically between two differently oriented electric fields, allows the separation of DNA molecules up to 5
  6. Experimental chemiluminescent detection of digoxin-labeled probes The digoxin system is over provided as another non-isotopic labeling method, which is detected by coupling an anti-digoxin antibody to one or more fluorescent d
  7. Detection of methylation by sequencing after bisulfite modification Bisulfite-modified sequencing can be used primarily to detect methylation.
  8. Expression experiments of multidrug resistance genes Expression experiments of multidrug resistance genes are mainly used for tumor therapy research.
  9. DEAE-dextran-mediated high-efficiency transfection assay Two forms of the classical DEAE-dextran transfection method are introduced here. The first one (the main option), in which the cells are exposed to high concent
  10. Bioparticle-mediated DNA transfection assay The following scheme is based on methods developed by Horch et al. (1999), Sanford et al. (1993), publications from major gene gun manufacturers (US/EG Bulletin
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