Cytosolic extracts can be used for splicing of precursor mRNAs. The S100 extract, although it includes many splicing factors, does not have splicing activity be
When Sanger and colleagues established the first DNA strand termination extension method, there was only one suitable DNA polymerase available, the Klenow fragm
Inoue's method (1990) for the preparation of E. coli receptor cells is good enough to achieve even the transformation efficiency of Hanahan's method (1980). How
This protocol is modeled after the method of Tabor and Richardson (1987a, 1989b, 1990), which uses a single-stranded DNA template for DNA sequencing (for single
The following method of denaturing plasmid DNA by base denaturation should be used in conjunction with Scheme 3, as sequencing enzymes are used to catalyze the
In the late 1970s and early 1980s, when Dong Hanahan was a graduate student at Cold Spring Harbor Laboratory and Harvard University, he made previously unheard
DNA cloning refers to the process of molecular manipulation in which a DNA fragment containing a target gene or other meaningful DNA is ligated in vitro with a
In the modification of DNA fragments, the dephosphorylation reaction is an important element, which is catalyzed by alkaline phosphatase, an enzyme that removes
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