Sulindac sulfide - 10mM in DMSO, high purity , CAS No.49627-27-2(DMSO)

Item Number

S656068

• Specifications & Purity: 10mM in DMSO
• Biochemical and Physiological Mechanisms: Sulindac sulfide is a noncompetitive γ-secretase inhibitor, with an IC 50 of 20.2 μM for γ 42 -secretase activity.
• Storage Temp: Store at -80°C
• Shipped In: Ice chest + Ice pads

Product Description

Sulindac sulfide is a noncompetitive γ-secretase inhibitor, with an IC 50 of 20.2 μM for γ 42 -secretase activity.

In Vitro

Treatment with 100 μM of Sulindac sulfide (SSide) causes cell death presumably by inducing apoptosis, resulting in marked decrease in Aβgeneration as well as in total protein expression. The IC 50 value for Aβ42 secretion of Sulindac sulfide is 30.6±2.8 μM. SSone and naproxen have no effect either on Aβ40 or Aβ42 secretion as well as on Notch cleavage up to 100 μM. It is observed that an inhibition of γ 42 -secretase activity by Sulindac sulfide in a dose-dependent manner. The IC 50 value of SSide for inhibiting γ 42 -secretase activity in vitro is 20.2±2.6 μM. A decrease is found in slope by the increase of the concentration of SSide in the plot of rate against the enzyme concentration, suggesting that Sulindac sulfide is not an irreversible or pseudo-irreversible inhibitor. Moreover, when the dialyzed solubilizedγ-secretase fraction is pretreated with Sulindac sulfide against CHAPSO buffer without Sulindac sulfide, γ-secretase activity is almost totally recovered. From these data, it is strongly suggested that the genuine molecular target of Sulindac sulfide is the γ-secretase complex and that Sulindac sulfide works as a reversible γ-secretase inhibitor. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

IC50& Target:IC50: 20.2 μM (γ 42 -secretase)