Stable red fluorophore probe. Widely used in cell labeling and Amplex Red type enzyme assays. It represents the reduced dye formed from Amplex Red reaction or resazurin reductions. Unstable in the presence of thiols such as dithiothreitol (DTT) and 2-merc
Storage Temp
Store at -20°C
Shipped In
Ice chest + Ice pads
Note
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Product Description
Red fluorescent group standard. It is widely used in enzyme determination system. The absorption and fluorescence of trihalogenase are pH dependent. The absorption transfer to about 480nm, e and fluorescence quantum yield decreased significantly. In the presence of thiols such as DTT and 2-mercaptoethanol, the experimental haloling is unstable.
The experimental halogenase has high extinction coefficient and good chemical and light stability. These properties of trihaloling make it very suitable for the detection of H2O2 by reverse fluorescence method. The fluorescence disappearance of horseradish peroxidase mediated oxidation into non fluorescent derivatives is determined.
1.Mengjing Zhao, Huan Zhao, Jiangming Deng, Lianxia Guo, Baojian Wu. (2019) Role of the CLOCK protein in liver detoxification. BRITISH JOURNAL OF PHARMACOLOGY, 176 (24):(4639-4652). [PMID:31404943][10.1111/bph.14828]
2.Xu Mao, Jian Wang, Qian Wang, Lan Yang, Yilin Li, Hao Lin, Ying Peng, Jiang Zheng. (2019) Nitidine Chloride–Induced CYP1 Enzyme Inhibition and Alteration of Estradiol Metabolism. DRUG METABOLISM AND DISPOSITION, 47 (8):(919-927). [PMID:31147316][10.1124/dmd.119.086892]
References
1.Mengjing Zhao, Huan Zhao, Jiangming Deng, Lianxia Guo, Baojian Wu. (2019) Role of the CLOCK protein in liver detoxification. BRITISH JOURNAL OF PHARMACOLOGY, 176 (24):(4639-4652). [PMID:31404943][10.1111/bph.14828]
2.Xu Mao, Jian Wang, Qian Wang, Lan Yang, Yilin Li, Hao Lin, Ying Peng, Jiang Zheng. (2019) Nitidine Chloride–Induced CYP1 Enzyme Inhibition and Alteration of Estradiol Metabolism. DRUG METABOLISM AND DISPOSITION, 47 (8):(919-927). [PMID:31147316][10.1124/dmd.119.086892]
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