Recombinant Rho A + B + C Antibody - Primary antibody, specific to RHOA; RHOB; RHOC, Rabbit IgG

    Application:
  • IF/ICC
  • IHC
Features and benefits
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab125253
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab125253-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab125253-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$209.90
Ab125253-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab125253-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,299.90

Recombinant; Rabbit anti Human Rho A + B + C Antibody; WB, IHC, ICC, IF; Unconjugated

Basic Description

Product NameRecombinant Rho A + B + C Antibody - Primary antibody, specific to RHOA; RHOB; RHOC, Rabbit IgG
SynonymsAA017882 antibody | AI324259, antibody | Aplysia ras related homolog 9 (RhoC) antibody | Aplysia ras-related homolog 12 antibody | Aplysia RAS-related homolog 6 antibody | Aplysia RAS-related homolog antibody | ARH12 antibody | ARH6 antibody | ARH9 antibo
Specifications & PurityRecombinant, ExactAb™, Validated, See COA
Host speciesRabbit
SpecificityRHOA; RHOB; RHOC
ImmunogenA synthesized peptide derived from human Rho A + B + C (AA 1-193)
Positive ControlWB: Mouse lung, Mouse kidney, Rat brain, HeLa, Jurkat, NIH/3T3 lysates. IHC: Human lung carcinoma tissue. ICC/IF: Hela cells.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human Rho A + B + C Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/2000
IHC: 1/500-1/1000
ICC/IF: 1/100-1/500
Protein Function
Rho A: Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP.
Rho B: Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development.
Rho C: Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.

AI Insight

Product Properties

IsotypeRabbit IgG
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
ConcentrationSee COA
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Rho A + B + C Antibody (Ab125253) - Western Blot
All lanes: Recombinant Rho A + B + C Antibody (Ab125253) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 23 kDa
Exposure time: 100.0 s

Recombinant Rho A + B + C Antibody (Ab125253) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant Rho A + B + C Antibody (Ab125253).

Recombinant Rho A + B + C Antibody (Ab125253) - IHC
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Recombinant Rho A + B + C Antibody (Ab125253).

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
IHC

1:500-1000

ICC/IF

1/100-1/500

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0708630Certificate of AnalysisJul 23, 2024 Ab125253
ZJ24F0708628Certificate of AnalysisJul 23, 2024 Ab125253

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