A synthesized peptide derived from human Rho A + B + C (AA 1-193)
Positive Control
WB: Mouse lung, Mouse kidney, Rat brain, HeLa, Jurkat, NIH/3T3 lysates. IHC: Human lung carcinoma tissue. ICC/IF: Hela cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human Rho A + B + C Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/2000 IHC: 1/500-1/1000 ICC/IF: 1/100-1/500 Protein Function Rho A: Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Rho B: Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Rho C: Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.
AI Insight
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
See COA
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Rho A + B + C Antibody (Ab125253) - Western Blot All lanes: Recombinant Rho A + B + C Antibody (Ab125253) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 22 kDa Observed band size: 23 kDa Exposure time: 100.0 s
Recombinant Rho A + B + C Antibody (Ab125253) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant Rho A + B + C Antibody (Ab125253).
Recombinant Rho A + B + C Antibody (Ab125253) - IHC Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Recombinant Rho A + B + C Antibody (Ab125253).
Mechanisms of Action
Mechanism of Action
Action Type
target ID
Target Name
Target Type
Target Organism
Binding Site Name
References
Application
Application
Dilution info
IHC
1:500-1000
ICC/IF
1/100-1/500
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Find and download the COA for your product by matching the lot number on the packaging.