Bioactivity: Specific Activity: ≥10U/mg pro. UNIT DEFINITION: Under the conditions of 25°C and pH 8.0, the amount of enzyme needed to catalyze the conversion of 1 μmol Boc-QR R-pNA into products per minute is one enzyme activity unit (U).
Endotoxin Concentration: <0.1 EU/μg
Item Number
R141093
Grouped product items
SKU
Size
Availability
Price
Qty
R141093-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$129.90
R141093-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$329.90
R141093-5mg
5mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$809.90
R141093-10mg
10mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
Kex2 is a Ca2+-dependent serine protease and cleaves at C-terminal site of Lys-Arg, Arg-Arg, Pro-Arg in pro-α,factor and killer-toxin precursors maturing, it was discovered in Saccharomyces cerevisiae.But Kex2 can’t recognize and cut a single basic amino acid,such as carboxyl end peptide bond of arginine and lysine. Recombinant Kex2 is a genetically engineered protein expressed in Pichia pastoris and purified by high pressure liquid chromatography. The optimal pH of Kex2 protease is 9.0, and the optimal temperature is 37 ℃. It is stable in buffer (pH 5.0-6.0). The activity of Kex2 is not affected by the conventional serine protease inhibitors such as PMSF, TPCK, TLCK inhibition. Kex2 Protease Recommended Method: Recommended reaction buffer: 5-10 mM Tris, 2mM Ca2+, pH 7.0-9.0 or HEPES, 5mM Ca2+, pH 7.0-9.0. If it is not used immediately after dissolution, it is recommended to use 20mM NaAc-HAc, 2mM Ca2+ (pH 5.0~5.5) buffer to dissolve the lyophilized powder. During the reaction, use 5-10 mM Tris, 2mM Ca2+, pH 7.0-9.0 or HEPES, 5mM Ca2+, pH 7.0-9.0 as the reaction diluent. Recommended conditions:
Catalytic activity: Cleavage of -Lys-Arg-|-Xaa- and -Arg-Arg-|-Xaa- bonds to process yeast alpha-factor pheromone and killer toxin precursors. Cofactor Ca2+. Note: Binds 3 Ca2+ ions per subunit.
Post-translational: O-glycosylated.
Bioactivity
Specific Activity: ≥10U/mg pro. UNIT DEFINITION: Under the conditions of 25°C and pH 8.0, the amount of enzyme needed to catalyze the conversion of 1 μmol Boc-QR R-pNA into products per minute is one enzyme activity unit (U).
Endotoxin Concentration
<0.1 EU/μg
Predicted molecular weight
80 kDa
SDS-PAGE
68-92 kDa
AI Insight
Product Specifications
Concentration
≥10 units/mg
Reconstitution
20mM NaAc-HAc, 2mM Ca2+, pH 5.0~5.5.
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Lyophilized powder store at -20℃ for 24 months. Dissolve in buffer (20mM NaAc-HAc, 2 mM Ca2+, pH 5.0~5.5), subpackage and store at -20℃. It is recommended that it should be stored at 2~8℃ after dissolution for no more than 24 hours. Upon receipt, it is re
