| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| G420549-1ml | 1ml | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $164.90 |
Aminoglycoside antibiotic agent
| Synonyms | 108321-42-2 | G418 Disulfate | Geneticin (G418 Sulfate) | G 418 disulfate salt | (2S,3S,4S,5S)-2-[(1S,2S,3R,4S,6R)-4,6-diamino-3-[(2S,3R,4R,5S,6R)-3-amino-4,5-dihydroxy-6-(1-hydroxyethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol |
|---|---|
| Specifications & Purity | 10mM in Water |
| Biochemical and Physiological Mechanisms | G-418 inhibits protein synthesis at the early stages of elongation, post-initiation, at the initiation stage of translation as well as just before the initiation stage of translation. |
| Storage Temp | Store at -80°C |
| Shipped In | Ice chest + Ice pads |
| Product Description | Selection agent for both prokaryotic and eukaryotic transfected cells. An aminoglycoside similar to gentamycin, G418 is toxic to bacterial, yeast, higher plant and mammalian cells, protozoans, and helminths. application： G418 disulfate is used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene. Optimal concentrations for selection and maintenance vary by cell lines. |
| IUPAC Name | (2S,3S,4S,5S)-2-[(1S,2S,3R,4S,6R)-4,6-diamino-3-[(2S,3R,4R,5S,6R)-3-amino-4,5-dihydroxy-6-(1-hydroxyethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol;sulfuric acid |
|---|---|
| INCHI | InChI=1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1 |
| InChi Key | UHEPSJJJMTWUCP-NKCAIAFTSA-N |
| Canonical SMILES | CC(C1C(C(C(C(O1)OC2C(CC(C(C2O)OC3C(C(C(CO3)(C)O)NC)O)N)N)N)O)O)O.OS(=O)(=O)O.OS(=O)(=O)O |
| Isomeric SMILES | CC([C@@H]1[C@H]([C@@H]([C@H]([C@H](O1)O[C@@H]2[C@H](C[C@H]([C@@H]([C@H]2O)O[C@H]3[C@H]([C@@H]([C@](CO3)(C)O)NC)O)N)N)N)O)O)O.OS(=O)(=O)O.OS(=O)(=O)O |
| WGK Germany | 3 |
| PubChem CID | 16218858 |
| Molecular Weight | 692.71 |
| Pictogram(s) | GHS08, GHS07 |
|---|---|
| Signal | Danger |
| Hazard Statements | H335:May cause respiratory irritation H373:Causes damage to organs through prolonged or repeated exposure H317:May cause an allergic skin reaction H334:May cause allergy or asthma symptoms or breathing difficulties if inhaled H360:May damage fertility or the unborn child |
| Precautionary Statements | P261:Avoid breathing dust/fume/gas/mist/vapors/spray. P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of water. P321:Specific treatment (see ... on this label). P405:Store locked up. P501:Dispose of contents/container to ... P260:Do not breathe dust/fume/gas/mist/vapors/spray. P284:[In case of inadequate ventilation] Wear respiratory protection. P271:Use only outdoors or in a well-ventilated area. P304+P340:IF INHALED: Remove person to fresh air and keep comfortable for breathing. P403+P233:Store in a well-ventilated place. Keep container tightly closed. P272:Contaminated work clothing should not be allowed out of the workplace. P333+P313:IF SKIN irritation or rash occurs: Get medical advice/attention. P362+P364:Take off contaminated clothing and wash it before reuse. P203:Obtain, read and follow all safety instructions before use. P318:if exposed or concerned, get medical advice. P319:Get medical help if you feel unwell. P342+P316:If experiencing respiratory symptoms: Get emergence medical help immediately. |
| WGK Germany | 3 |
| 1. Han Sijia, Jin Xueying, Hu Tianyu, Chi Feng. (2023) ARHGAP25 suppresses the development of breast cancer by an ARHGAP25/Wnt/ASCL2 feedback loop. CARCINOGENESIS, [PMID:37326327] [10.1093/carcin/bgad042] |
| 2. Sijia Han, Xueying Jin, Tianyu Hu, Feng Chi. (2023) LAPTM5 regulated by FOXP3 promotes the malignant phenotypes of breast cancer through activating the Wnt/β‑catenin pathway. ONCOLOGY REPORTS, 49 (3): (1-14). [PMID:36799186] [10.3892/or.2023.8497] |
| 3. Jing Zhou, Tongyu Wu, Chun Li, Zhuozhou Hu, Liang Han, Xiangxiang Li, Jingjing Liu, Wenyang Zhao, Jia Kang, Xinping Chen. (2023) Alfuzosin ameliorates diabetes by boosting PGK1 activity in diabetic mice. LIFE SCIENCES, 317 (121491). [PMID:36758669] [10.1016/j.lfs.2023.121491] |
| 4. Zhuozhou Hu, Jing Zhou, Liang Han, Xiangxiang Li, Chun Li, Tongyu Wu, Jingjing Liu, Wenyang Zhao, Jia Kang, Xinping Chen. (2022) Acyclovir alleviates insulin resistance via activating PKM1 in diabetic mice. LIFE SCIENCES, 304 (120725). [PMID:35751919] [10.1016/j.lfs.2022.120725] |
| 5. Jie Zhu, Kun Yang, Aijie Liu, Xiaoxue Lu, Linsong Yang, Qinghuan Zhao. (2020) Highly secretory expression of recombinant cowpea chlorotic mottle virus capsid proteins in Pichia pastoris and in-vitro encapsulation of ruthenium nanoparticles for catalysis. PROTEIN EXPRESSION AND PURIFICATION, 174 (105679). [PMID:32534017] [10.1016/j.pep.2020.105679] |
| 6. 王帅. (2024) SOX4 facilitates brown fat development and maintenance through EBF2-mediated thermogenic gene program in mice. Cell Death & Differentiation, 12 (9): (1407). [PMID:39402212] [https://doi.org/10.1038/s41418-024-01397-0] |
| 1. Han Sijia, Jin Xueying, Hu Tianyu, Chi Feng. (2023) ARHGAP25 suppresses the development of breast cancer by an ARHGAP25/Wnt/ASCL2 feedback loop. CARCINOGENESIS, [PMID:37326327] [10.1093/carcin/bgad042] |
| 2. Sijia Han, Xueying Jin, Tianyu Hu, Feng Chi. (2023) LAPTM5 regulated by FOXP3 promotes the malignant phenotypes of breast cancer through activating the Wnt/β‑catenin pathway. ONCOLOGY REPORTS, 49 (3): (1-14). [PMID:36799186] [10.3892/or.2023.8497] |
| 3. Jing Zhou, Tongyu Wu, Chun Li, Zhuozhou Hu, Liang Han, Xiangxiang Li, Jingjing Liu, Wenyang Zhao, Jia Kang, Xinping Chen. (2023) Alfuzosin ameliorates diabetes by boosting PGK1 activity in diabetic mice. LIFE SCIENCES, 317 (121491). [PMID:36758669] [10.1016/j.lfs.2023.121491] |
| 4. Zhuozhou Hu, Jing Zhou, Liang Han, Xiangxiang Li, Chun Li, Tongyu Wu, Jingjing Liu, Wenyang Zhao, Jia Kang, Xinping Chen. (2022) Acyclovir alleviates insulin resistance via activating PKM1 in diabetic mice. LIFE SCIENCES, 304 (120725). [PMID:35751919] [10.1016/j.lfs.2022.120725] |
| 5. Jie Zhu, Kun Yang, Aijie Liu, Xiaoxue Lu, Linsong Yang, Qinghuan Zhao. (2020) Highly secretory expression of recombinant cowpea chlorotic mottle virus capsid proteins in Pichia pastoris and in-vitro encapsulation of ruthenium nanoparticles for catalysis. PROTEIN EXPRESSION AND PURIFICATION, 174 (105679). [PMID:32534017] [10.1016/j.pep.2020.105679] |
| 6. 王帅. (2024) SOX4 facilitates brown fat development and maintenance through EBF2-mediated thermogenic gene program in mice. Cell Death & Differentiation, 12 (9): (1407). [PMID:39402212] [https://doi.org/10.1038/s41418-024-01397-0] |
