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Basic Description
| Synonyms | γ-[(6-Azidohexyl)-imido]-adenosine-5’-triphosphate sodium salt |
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| Legal Information | The purchase of this product for use in applications relating to copper catalyzed azide-alkyne cycloaddition chemistry ("Click Chemistry") includes a limited, nontransferable license to intellectual property owned by The Scripps Research Institu |
| Storage Temp | Store at -20°C |
| Shipped In | Ice chest + Ice pads |
| Product Description | Description Lee, et al., reported a non-radioactive version ofin vitrophosphorylation where γ-[(6-Azidohexyl)-imido]-ATP (compound 6) was successfully used instead of γ-32P-modified ATP to phosphorylate GST-tagged recombinant p27kip1 with protein kinase cdk2. The phosphorylated, azide-modified protein substrate can be labeled with alkynes of biotin or fluorescent dyes using Cu(I)-catalyzed click chemistry or DBCO-containing biotin or fluorescent dyes using Cu(I)-free click chemistry. |
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Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Chemical and Physical Properties
| Solubility | 10 mM Tris-HCl, pH 7.5: soluble |
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References
| 1. Sarah E Lee, Lucy M Elphick, Alexandra A Anderson, Laurent Bonnac, Emma S Child, David J Mann, Véronique Gouverneur,. (2009-05-05) Synthesis and reactivity of novel gamma-phosphate modified ATP analogues.. Bioorganic & medicinal chemistry letters, 19 ((14)): ( 3804-3807 ). [PMID:19410453] |