Ac-DEVD-AFC - BioReagent, ≥98% , CAS No.201608-14-2

CAS: 201608-14-2 Cat. No.: A769903 Molecular Weight: 729.61

GRADE & PURITY BioReagent, ≥98%

Synonyms

N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin | NAc-Asp-Glu-Val-Asp-AFC | Ac-Asp-Glu-Val-Asp-AFC

Storage

Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

Apoptosis, Cell signaling, Drug screening, Enzyme activity assay

Overview

A fluorogenic substrate for caspase 3, a protease that is rapidly activated when cells are exposed to apoptotic conditions and that cleaves poly(ADP-ribose) polymerase. The 7-amido-4-trifluoromethylcoumarin (AFC) derivative has better membrane permeability than the 7-amido-4-methylcoumarin (AMC) derivative.A fluorogenic substrate for caspase 3; has better membrane permeability than the 7-amido-4-methylcoumarin derivative.

Specifications

Synonyms

N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin | NAc-Asp-Glu-Val-Asp-AFC | Ac-Asp-Glu-Val-Asp-AFC

Specifications & Purity

BioReagent, ≥98%

Stability And Storage

Store at -20℃ long term (24 months).  Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark. Desiccated.

Storage

Protected from light, Store at -20°C, Desiccated, Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent

Purity

≥98%

Names and Identifiers

• Canonical Smiles: CC(C)C(C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C(CCC(=O)O)NC(=O)C(CC(=O)O)NC(=O)C
• IUPAC Name: (4S)-4-[[(2S)-2-acetamido-3-carboxypropanoyl]amino]-5-[[(2S)-1-[[(2S)-3-carboxy-1-oxo-1-[[2-oxo-4-(trifluoromethyl)chromen-7-yl]amino]propan-2-yl]amino]-3-methyl-1-oxobutan-2-yl]amino]-5-oxopentanoic acid
• InChIKey: GZDRODOYEFEHGG-NUDCOPPTSA-N
• INCHI: 1S/C30H34F3N5O13/c1-12(2)25(38-26(47)17(6-7-21(40)41)36-28(49)18(10-22(42)43)34-13(3)39)29(50)37-19(11-23(44)45)27(48)35-14-4-5-15-16(30(31,32)33)9-24(46)51-20(15)8-14/h4-5,8-9,12,17-19,25H,6-7,10-11H2,1-3H3,(H,34,39)(H,35,48)(H,36,49)(H,37,50)(H,38,47)(H,40,41)(H,42,43)(H,44,45)/t17-,18-,19-,25-/m0/s1
• Isomeric SMILES: CC(C)[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)C
• Molecular Weight: 729.61

Chemical and Physical Properties

• Solubility: Soluble in 20% ACN/H2O.
• Molecular Weight: 729.600 g/mol
• XLogP3: -0.800
• Hydrogen Bond Donor Count: 8
• Hydrogen Bond Acceptor Count: 16
• Rotatable Bond Count: 17
• Exact Mass: 729.211 Da
• Monoisotopic Mass: 729.211 Da
• Topological Polar Surface Area: 284.000 Ų
• Heavy Atom Count: 51
• Formal Charge: 0
• Complexity: 1430.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 4
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1