GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.0.5 mg/mL
ExactAb™, Validated, Azide Free, 0.5 mg/mL Azide Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Application:
Flow: ≤1μg/test
For flow cytometric staining, the suggested use of this reagent is ≤1 μg per million cells in 100 µL staining volume or 100 µL of whole blood.
Specifications
Product Name
Rat IgG (Biotin) - Isotype Control Antibody, IgG
Synonyms
Rat IgG | Biotin-conjugated Rat Polyclonal antibody | Biotin Rat IgG - Isotype Control
Specifications & Purity
ExactAb™, Validated, Azide Free, 0.5 mg/mL
Host species
Rat
Conjugation
Biotin
Grade
Azide Free, ExactAb™, Validated
Isotype
IgG
Purification Method
Affinity chromatography
Concentration
0.5 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images
Rat IgG Isotype Control Antibody (Biotin) (Ab180310) - ELISA Rat IgG Isotype Control Antibody (Biotin) (Ab180310) was tested by direct ELISA. The plate was coated with Goat Anti-Rat IgG H&L Antibody (Ab138352). A serial dilution of Rat IgG Isotype Control Antibody (Biotin) (Ab180310) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.
Rat IgG Isotype Control Antibody (Biotin) (Ab180310) - Flow Cytometry Flow cytometry analysis of CD16/CD32 expression by mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD16/CD32 Rat mAb (Biotin) (Ab179681) at 0.03μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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