Fecal specimen processing test
The fecal specimen taken directly affects the accuracy of the results, usually using naturally excreted feces, specimen collection precautions are as follows:
1. Fecal test should take fresh specimens, clean containers, not mixed with urine, no disinfectant and sewage, so as not to destroy the formation of components, so that the death of pathogenic bacteria and contamination of putrefactive protozoa.
2. When collecting specimens, clean bamboo sticks should be used to select feces containing mucus, pus and blood and other lesions; feces without abnormal appearance should be taken from the surface, depth and fecal end of the feces from many places, and the amount of feces should be at least the size of a fingertip.
3. The specimen should be examined within 1 hour after collection.
4. Dysentery amebic trophozoites should be examined immediately after defecation. The specimen should be taken from the pus and blood and the thin and soft part of the feces, and the specimen should be kept warm during transmission and examination in the cold season.
5. For examination of Schistosoma haematobium eggs, mucus, pus and blood should be taken, and at least 30 grams of feces should be retained for incubation of trichinella, and should be processed as soon as possible.
6. The eggs of pinworms should be examined by swabbing with transparent film at 12 o'clock in the evening or in the morning before defecation from the perianal fissure and immediately examined microscopically.
7. 24-hour feces should be collected for finding parasitic worms and for egg counting; the former should be carefully searched or sieved from all the feces and then identified to its species; the latter should be mixed and examined.
8. For chemical occult blood test, meat and food containing animal blood should be prohibited for three days before, and iron and vitamin C should be prohibited.
9. For quantification of fecal bile, feces should be collected for 3 consecutive days, and 20 grams of feces should be taken out every day after homogenization and weighing for examination.
10. Stool specimens for bacteriological examination should be collected in sterilized containers with lids and sent for examination immediately.
11. When there is no fecal discharge but examination is necessary, specimen can be swabbed by anal finger-pointing or stool collection tube. The feces from enema or oil laxative is often too dilute and thirsty for oil droplets, which is unsuitable for examination specimen.
12. Paper or plastic specimen boxes should be burned in an incinerator after fecal examination. Enamel containers should be soaked in disinfectant solution (such as peroxyacetic acid, coal phenol soap or neosporin, etc.) for 24 hours, after discarding the disinfectant solution, rinse with running water for spare.
Operation method
Fecal specimen processing test Move 1. Cultivation: For more product details, please visit Aladdin Scientific website.
Salmonella-Shigella: Inoculate SS and Chinese Orchid or MacConkey, Irhonmelan plate, check the plate after 18-24 hours at 35℃, if no suspected colonies of Salmonella or Shigella are found, repeat the check once in 48 hours. If no suspicious colonies are found, it can be reported as "Salmonella (or Shigella) not detected"; if there are suspected colonies of the two genera, the preliminary biochemical reaction is consistent with, and obvious agglutination with one of the diagnostic sera of Shigella, it can be reported as "××Shigella detected"; if there is agglutination with the diagnostic sera of Salmonella multivalent and group factor, it can only be reported as "×-group Salmonella detected"; if there is a condition of identification of sera of Shigella typing or Salmonella factor sera, and the condition is consistent with, then it can be reported as "×-group Salmonella". If there are conditions to be identified by Shigella typing serum or Salmonella factor serum, and in accordance with the antigenic formula of the bacterium, it can be reported as "detection of ×× Shigella × type" or "detection of ×× Salmonella".
2. Reporting method:
It has been found that a variety of bacteria can cause diarrhea, and many of them require special culture. When isolating pathogenic bacteria in feces, the negative results of culture should not be reported as "no pathogenic bacteria" or "no pathogenic bacteria detected" if the necessary conditions for the growth of the above types of bacteria are not provided.
The mode of reporting should be determined by the results of isolation of the target organisms, e.g., the current routine isolation of pathogenic bacteria from feces by SS and Chinese or Erythromycin, positive results should be reported as "Salmonella or Shigella" and negative results should be reported as "Salmonella spp. and Shigella spp. not detected". Other culture results should be reported in the same way as the above principles.