| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| C278976-100mg | 100mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,399.90 |
Tissue dissociators, dissociated tissue and monolayer cells
| Product Name | Collagenase NB 6 From Clostridium Histolyticum - ≥ 0.100 U/mg, high purity , CAS No.9001-12-1 |
|---|---|
| Synonyms | Collagenase I | Collagenase Type II | (5S,6S,9R,10S,13S,17S,23S,24S,27R,28S,31S,35S)-5,6,9,13,17,23,24,27,31,35-Decamethyl-10,28-dioctyl-2,20-diazanonacyclo[19.15.0.03,19.05,17.06,14.09,13.023,35.024,32.027,31]hexatriaconta-1(21),2,19-triene |
| Grade | GMP, EnzymoPure™ |
| Product Description | Collagenase NB 6 is a crude collagenase that contains collagenolytic and additional enzymatic activities including clostripain and neutral protease. The balanced ratio of these activities ensures gentle and efficient tissue dissociation. Specifications Contains class I and class II collagenase (1) and a balanced ratio of proteolytic side activities. Activity (Wünsch): ≥ 0.100 U/mg Microbiological examination: Sterility, absence of Clostridia, abnormal toxicity (general safety test; Ph. Eur.). Sterile according to Ph. Eur. (Clostridiopeptidase A) EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1] Applications Collagenase NB 6 is suitable for cell isolation from various tissue types intended for clinical applications. References and Definitions Unit definition: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1. References 1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091 2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51 |
| Specifications & Purity | EnzymoPure™, GMP, ≥ 0.100 U/mg |
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Concentration | ≥ 0.100 U/mg |
|---|---|
| Storage Temp | Store at 2-8°C |
| Shipped In | Wet ice |
| CAS | 9001-12-1 |
| Enzyme Commission Number | 3.4.24.3 |
| Unit definition | 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1. |
| Pictogram(s) | GHS08 |
|---|---|
| Signal | Warning |
| Hazard Statements | H334:May cause allergy or asthma symptoms or breathing difficulties if inhaled |
| Precautionary Statements | P261:Avoid breathing dust/fume/gas/mist/vapors/spray. P501:Dispose of contents/container to ... P284:[In case of inadequate ventilation] Wear respiratory protection. |
| WGK Germany | 1 |
| 1. Jie Cao, Xiaoxuan Du, Hui Zhao, Chenhong Zhu, Chenchen Li, Xin Zhang, Libin Wei, Xue Ke. (2023) Sequentially degradable hydrogel-microsphere loaded with doxorubicin and pioglitazone synergistically inhibits cancer stemness of osteosarcoma. BIOMEDICINE & PHARMACOTHERAPY, 165 (115096). [PMID:37421781] [10.1016/j.biopha.2023.115096] |
| 2. Meiqi Jin, Ningning Sun, Wenxian Weng, Zhentao Sang, Taotao Liu, Wei Xia, Shuze Wang, Xiaoting Sun, Tianlin Wang, Heran Li, Huazhe Yang. (2023) The effect of GelMA/alginate interpenetrating polymeric network hydrogel on the performance of porous zirconia matrix for bone regeneration applications. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 242 (124820). [PMID:37178890] [10.1016/j.ijbiomac.2023.124820] |
| 3. Lanting Wei, Xiang Wang, Junjie Fu, Jian Yin, Jing Hu. (2023) A physically cross-linked double network polysaccharides/Ca2+ hydrogel scaffold for skeletal muscle tissue engineering. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 668 (131410). [PMID:] [10.1016/j.colsurfa.2023.131410] |
| 4. Hongqian Zhang, Feng Ding, Zongwei Zhu, Qian Sun, Chuanxu Yang. (2023) Engineered ionizable lipid nanoparticles mediated efficient siRNA delivery to macrophages for anti-inflammatory treatment of acute liver injury. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 631 (122489). [PMID:36521639] [10.1016/j.ijpharm.2022.122489] |
| 5. Zunhan Hu, Wenzhen Wu, Meizhe Yu, Zhi Wang, Zhenyu Yang, Xiaodong Xing, Xiaofang Chen, Lina Niu, Fan Yu, Yuhong Xiao, Jihua Chen. (2023) Mussel-inspired polymer with catechol and cationic Lys functionalities for dentin wet bonding. Materials Today Bio, 18 (100506). [PMID:36471892] [10.1016/j.mtbio.2022.100506] |
| 6. Wanmei Zhou, Xueming Dong, Yang He, Wei Zheng, Jinsong Leng. (2022) In-vitro and in-vivo studies of 4D printed shape memory scaffolds with bioactive fillers and coating for enhanced bone tissue regeneration. Smart Materials and Structures, 31 (10): (105002). [PMID:] [10.1088/1361-665X/ac884a] |
| 7. Qian Ji, Hao Zhang, Xihe Zhang, Qiaoqiao Ma, Liping Teng, Lipeng Qiu. (2020) Hydrosoluble collagen based biodegradable hybrid hydrogel for biomedical scaffold. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 31 (17): (2199-2219). [PMID:32663418] [10.1080/09205063.2020.1796229] |
| 8. Guangxiu Cao, Cunyang Wang, Yubo Fan, Xiaoming Li. (2020) Biomimetic SIS-based biocomposites with improved biodegradability, antibacterial activity and angiogenesis for abdominal wall repair. Materials Science & Engineering C-Materials for Biological Applications, 109 (110538). [PMID:32228945] [10.1016/j.msec.2019.110538] |
| 9. Dajiang Kuang, Fujian Jiang, Feng Wu, Kulwinder Kaur, Sourabh Ghosh, Subhas C. Kundu, Shenzhou Lu. (2019) Highly elastomeric photocurable silk hydrogels. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 134 (838). [PMID:31103592] [10.1016/j.ijbiomac.2019.05.068] |
| 10. Huiyan Ying, Juan Zhou, Mingyu Wang, Dandan Su, Qiaoqiao Ma, Guozhong Lv, Jinghua Chen. (2019) In situ formed collagen-hyaluronic acid hydrogel as biomimetic dressing for promoting spontaneous wound healing. Materials Science & Engineering C-Materials for Biological Applications, 101 (487). [PMID:31029343] [10.1016/j.msec.2019.03.093] |
| 11. Juan Zhou, Huiyan Ying, Mingyu Wang, Dandan Su, Guozhong Lu, Jinghua Chen. (2018) Dual layer collagen-GAG conduit that mimic vascular scaffold and promote blood vessel cells adhesion, proliferation and elongation. Materials Science & Engineering C-Materials for Biological Applications, 92 (447). [PMID:30184770] [10.1016/j.msec.2018.06.072] |
| 1. Jie Cao, Xiaoxuan Du, Hui Zhao, Chenhong Zhu, Chenchen Li, Xin Zhang, Libin Wei, Xue Ke. (2023) Sequentially degradable hydrogel-microsphere loaded with doxorubicin and pioglitazone synergistically inhibits cancer stemness of osteosarcoma. BIOMEDICINE & PHARMACOTHERAPY, 165 (115096). [PMID:37421781] [10.1016/j.biopha.2023.115096] |
| 2. Meiqi Jin, Ningning Sun, Wenxian Weng, Zhentao Sang, Taotao Liu, Wei Xia, Shuze Wang, Xiaoting Sun, Tianlin Wang, Heran Li, Huazhe Yang. (2023) The effect of GelMA/alginate interpenetrating polymeric network hydrogel on the performance of porous zirconia matrix for bone regeneration applications. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 242 (124820). [PMID:37178890] [10.1016/j.ijbiomac.2023.124820] |
| 3. Lanting Wei, Xiang Wang, Junjie Fu, Jian Yin, Jing Hu. (2023) A physically cross-linked double network polysaccharides/Ca2+ hydrogel scaffold for skeletal muscle tissue engineering. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 668 (131410). [PMID:] [10.1016/j.colsurfa.2023.131410] |
| 4. Hongqian Zhang, Feng Ding, Zongwei Zhu, Qian Sun, Chuanxu Yang. (2023) Engineered ionizable lipid nanoparticles mediated efficient siRNA delivery to macrophages for anti-inflammatory treatment of acute liver injury. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 631 (122489). [PMID:36521639] [10.1016/j.ijpharm.2022.122489] |
| 5. Zunhan Hu, Wenzhen Wu, Meizhe Yu, Zhi Wang, Zhenyu Yang, Xiaodong Xing, Xiaofang Chen, Lina Niu, Fan Yu, Yuhong Xiao, Jihua Chen. (2023) Mussel-inspired polymer with catechol and cationic Lys functionalities for dentin wet bonding. Materials Today Bio, 18 (100506). [PMID:36471892] [10.1016/j.mtbio.2022.100506] |
| 6. Wanmei Zhou, Xueming Dong, Yang He, Wei Zheng, Jinsong Leng. (2022) In-vitro and in-vivo studies of 4D printed shape memory scaffolds with bioactive fillers and coating for enhanced bone tissue regeneration. Smart Materials and Structures, 31 (10): (105002). [PMID:] [10.1088/1361-665X/ac884a] |
| 7. Qian Ji, Hao Zhang, Xihe Zhang, Qiaoqiao Ma, Liping Teng, Lipeng Qiu. (2020) Hydrosoluble collagen based biodegradable hybrid hydrogel for biomedical scaffold. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 31 (17): (2199-2219). [PMID:32663418] [10.1080/09205063.2020.1796229] |
| 8. Guangxiu Cao, Cunyang Wang, Yubo Fan, Xiaoming Li. (2020) Biomimetic SIS-based biocomposites with improved biodegradability, antibacterial activity and angiogenesis for abdominal wall repair. Materials Science & Engineering C-Materials for Biological Applications, 109 (110538). [PMID:32228945] [10.1016/j.msec.2019.110538] |
| 9. Dajiang Kuang, Fujian Jiang, Feng Wu, Kulwinder Kaur, Sourabh Ghosh, Subhas C. Kundu, Shenzhou Lu. (2019) Highly elastomeric photocurable silk hydrogels. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 134 (838). [PMID:31103592] [10.1016/j.ijbiomac.2019.05.068] |
| 10. Huiyan Ying, Juan Zhou, Mingyu Wang, Dandan Su, Qiaoqiao Ma, Guozhong Lv, Jinghua Chen. (2019) In situ formed collagen-hyaluronic acid hydrogel as biomimetic dressing for promoting spontaneous wound healing. Materials Science & Engineering C-Materials for Biological Applications, 101 (487). [PMID:31029343] [10.1016/j.msec.2019.03.093] |
| 11. Juan Zhou, Huiyan Ying, Mingyu Wang, Dandan Su, Guozhong Lu, Jinghua Chen. (2018) Dual layer collagen-GAG conduit that mimic vascular scaffold and promote blood vessel cells adhesion, proliferation and elongation. Materials Science & Engineering C-Materials for Biological Applications, 92 (447). [PMID:30184770] [10.1016/j.msec.2018.06.072] |
