ATTO 655

Item Number

A664947

• Storage Temp: Protected from light,Store at -20°C,Desiccated
• Shipped In: Ice chest + Ice pads

Product Description

Product Number	Product specifications	package		Product Number	Product specifications	package
A664947-1-500μg	Modification: tetrazine (MeTet) NEW	500μg		A664947-6-10nmol	Modification: phalloidin	10nmol
A664947-1-200μg	Modification: tetrazine (MeTet) NEW	200μg		A664947-7-5mg	Modification: biotin	5mg
A664947-2-5mg	Modification: alkyne	5mg		A664947-7-1mg	Modification: biotin	1mg
A664947-2-1mg	Modification: alkyne	1mg		A664947-8-5mg	Modification: streptavidin	5mg
A664947-3-5mg	Modification: iodoacetamide	5mg		A664947-8-1mg	Modification: streptavidin	1mg
A664947-3-1mg	Modification: iodoacetamide	1mg		A664947-9-5mg	Modification: maleimide	5mg
A664947-4-5mg	Modification: azide	5mg		A664947-9-1mg	Modification: maleimide	1mg
A664947-4-1mg	Modification: azide	1mg		A664947-10-5mg	Modification: NHS-ester	5mg
A664947-5-5mg	Modification: amine	5mg		A664947-10-1mg	Modification: NHS-ester	1mg
A664947-5-1mg	Modification: amine	1mg		A664947-11-5mg	Modification: free acid	5mg
A664947-6-20nmol	Modification: phalloidin	20nmol		A664947-11-1mg	Modification: free acid	1mg

Optical Properties

λabs	663nm
εmax	1.25×10⁵M⁻¹ cm⁻¹
λfl	680nm
ηfl	30%
τfl	1.8ns
CF₂₆₀=ε₂₆₀/εmax	0.24
CF₂₈₀=ε₂₈₀/εmax	0.08

ATTO 655 belongs together with ATTO 680 und ATTO 700 to a new generation of fluorescent labels. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high thermal and photo-stability, exceptionally high stability towards atmospheric ozone , and very good water solubility. Thus ATTO 655 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. ATTO 655 is a zwitterionic dye. After coupling to a substrate the dye moiety is electrically neutral. ATTO 655 is a strong electron acceptor. Its fluorescence is efficiently quenched by electron donors like guanine, tryptophan, etc. The fluorescence is excited most efficiently in the range 640 - 660 nm. A suitable excitation source is the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm.